SUMMARYAlx1, a member of the Cart1/Alx3/Alx4 subfamily of Paired-class homeodomain proteins, is an essential component of the gene network controlling skeletogenic fate specification in the sea urchin embryo

SummaryDifferential stability of β-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled

The endoskeleton of the sea urchin larva is a network of calcareous rods secreted by primary mesenchyme cells (PMCs). In this study, we identified seven new biomineralization-related proteins through an analysis of a large database of gene products expressed by PMCs. The proteins include three new spicule matrix proteins (SpSM29, SpSM32, and SpC-lectin), two proteins related to the PMC-specific cell surface glycoprotein MSP130 (MSP130-related-1 and -2), and two novel proteins (SpP16 and SpP19). The genes encoding these proteins are expressed specifically by cells of the large micromere-PMC lineage and are activated zygotically beginning at the blastula stage, prior to PMC ingression. Several of the mRNAs show regulated patterns of expression within the PMC syncytium that correlate with the pattern of skeletal rod growth. This work identifies new proteins that may regulate the process of biomineralization in this tractable model system.

The migratory primary mesenchyme cells (PMCs) of the sea urchin embryo are a model experimental system for the analysis of cell-extracellular matrix (ECM) interactions. Although the behavior of PMCs during gastrulation has been analyzed in considerable detail, it has proven difficult to identify specific substrate molecules with which these cells interact. Here, using a new monoclonal antibody (2.5C4) generated by an in vitro immunization procedure, we show that migrating PMCs interact with a distinct class of ECM fiber. The 2.5C4-positive fibers are distributed in a vegetal (high) to animal (low) gradient on the basal surface of the ectoderm. Three observations indicate that PMC filopodia interact directly with the fibers: (1) During gastrulation, 2.5C4-positive fibers gradually become oriented in a prominent circumferential belt that corresponds precisely to the position of the subequatorial PMC ring. (2) This fiber pattern is blocked by microsurgical removal of PMCs but is restored if PMCs are reintroduced into the embryo. (3) Examination of immunostained embryo whole mounts by confocal microscopy reveals a striking association between PMC filopodial roots and foci of fiber bundling. Double-immunostaining experiments using 2.5C4 and antibodies against previously identified matrix constituents show that the protein ECM3 is a component of the fibers. We have determined the complete amino acid sequence of ECM3 and find that this large protein (3103 amino acids) consists of an N-terminal domain similar to the mammalian chondroitin sulfate proteoglycan core protein NG2, a central region composed of five tandem repeats of a domain contained within the regulatory Ca2+-binding loop of Na+-Ca2+ exchange proteins, and a C-terminal region with no homology to known proteins. The general structure of ECM3 is similar in several respects to that of a sponge protein, MAFp4. MAFp4 is a major component of aggregation factor, an ECM complex that mediates the calcium-dependent, species-specific sorting of sponge cells. These studies establish ECM3 as a strong candidate for a PMC substrate molecule and point to several possible mechanisms by which interactions between PMC filopodia and ECM3-containing fibers could provide guidance information to migrating PMCs.

Wnt proteins and their receptors, members of the frizzled protein family, play a key role in regulating a wide range of developmental processes. Recently, putative regulators of Wnt signaling known as secreted frizzled-related proteins (SFRPs) have been identified in several vertebrates. Here, we describe the cloning of a novel SFRP (suSFRP1) from the sea urchin, Strongylocentrotus purpuratus. SuSFRP1 contains a putative signal sequence, four cysteine-rich domains and a single Ig domain. The developmental expression of suSFRP1 mRNA is highly dynamic and can be separated into three phases: (1) abrupt accumulation in most or all cells of the embryo at the early blastula stage; (2) restriction of expression to the prospective endoderm and animal pole region of the gastrula; and (3) expression in prospective muscle cells of the coelomic pouches during late embryogenesis.