The intergenic spacer (IGS) region of rDNA in Olea europaea is 5,629 bp long and it can be subdivided, on the basis of the different features, into six regions; two of them, located upstream and downstream of the putative transcription initiation site (TIS), respectively, contain prominent and unrelated subrepeats (s.r.). The upstream s.r. are 75-86 bp in length and the downstream ones are about 160 bp in length. The first type is represented by a high number (36) of iterations, which share a mutual similarity of 82.5%. A single sequence (TATTATAGGGGGGAGG) is found, which fits the reported TIS of plants and the position +1 would correspond to the adenine at position 3,622 after the 3′ end of 25 S ribosomal RNA (rDNA) genes. Moreover, in Olea IGS region some unusual features are observed and discussed in relation to analogous data present in the literature.
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