Abstract. Spiking Neuron Networks (SNNs) overcome the computational power of neural networks made of thresholds or sigmoidal units. Indeed, SNNs add a new dimension, the temporal axis, to the representation capacity and the processing abilities of neural networks. In this paper, we present how SNN can be applied with efficacy for cell microscopic image segmentation. Results obtained confirm the validity of the approach. The strategy is performed on cytological color images. Quantitative measures are used to evaluate the resulting segmentations.
Broncho alveolar lavage is the most commonly used diagnostic tool for confirming alveolar hemorrhage. Golde has introduced a ranking score, based on the hemosiderin content of macrophages which enables ranking cells from 0 to 4 based on the degree of Prussian blue stain. We propose a complete image analysis scheme to automatically perform both the extraction of the cellular objects and the ranking of each cell according to the Golde score. The image analysis techniques used mainly involve clustering and mathematical morphology. A 2D histogram is clustered to extract the main cellular components, a color watershed is used to determine and refine the regions. Finally, the cellular components of interest are firstly classified according to their hue and secondly according to their staining repartition. The proposed image analysis technique is very fast and produces reliable and accurate results.
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