Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for bla, bla and bla and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
Klebsiella species frequently cause clinically relevant human infections worldwide. We report the draft genome sequence of a Brazilian clinical isolate (Bz19) of the recently recognized species Klebsiella variicola. The comparison of Bz19 genome content with the At-22 (environmental K. variicola) and several clinical Klebsiella pneumoniae shows that these species share a set of virulence-associated determinants. Of note, this K. variicola strain harbours a plasmid-like element that shares the same backbone present in a multidrug-resistant plasmid found in a clinical K. pneumoniae isolated in USA.
There has been a resurgence in the number of pertussis cases in Brazil and around the
world. Here, the genome of a clinical Bordetella pertussis strain (Bz181) that was
recently isolated in Brazil is reported. Analysis of the virulence-associated genes
defining the pre- and post-vaccination lineages revealed the presence of the
prn2-ptxS1A-fim3B-ptxP3 allelic profile in Bz181, which is characteristic of the
current pandemic lineage. A putative metallo-β-lactamase gene presenting all of the
conserved zinc-binding motifs that characterise the catalytic site was identified, in
addition to a multidrug efflux pump of the RND family that could confer resistance to
erythromycin, which is the antibiotic of choice for treating pertussis disease.
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