Glutaraldehyde possesses unique characteristics that render it one of the most effective protein crosslinking reagents. It can be present in at least 13 different forms depending on solution conditions such as pH, concentration, temperature, etc. Substantial literature is found concerning the use of glutaraldehyde for protein immobilization, yet there is no agreement about the main reactive species that participates in the crosslinking process because monomeric and polymeric forms are in equilibrium. Glutaraldehyde may react with proteins by several means such as aldol condensation or Michael-type addition, and we show here 8 different reactions for various aqueous forms of this reagent. As a result of these discrepancies and the unique characteristics of each enzyme, crosslinking procedures using glutaraldehyde are largely developed through empirical observation. The choice of the enzyme-glutaraldehyde ratio, as well as their final concentration, is critical because insolubilization of the enzyme must result in minimal distortion of its structure in order to retain catalytic activity. The purpose of this paper is to give an overview of glutaraldehyde as a crosslinking reagent by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
A new method to reconstruct the elastic modulus of soft tissue subjected to an external static compression is presented. In this approach the Newton-Raphson method is used to vary a finite element (FE) model of the elasticity equations to fit, in a least squared sense, a set of axial tissue displacement fields estimated using a correlation technique applied to ultrasound signals. The ill-conditioning of the Hessian matrix is eliminated using the Tikhonov regularization technique. This regularization provides a compromise between fidelity to the observed data and a priori information of the solution. Using an echographic image formation model, it is shown that the method converges within a few iterations (8-10) and that strain images artifacts which are common in elastography are significantly reduced after the resolution of the inverse problem.
A theoretical model was previously developed to evaluate the relationship between the dynamics of ultrasonic speckle and its underlying tissue. The model is divided into an instrumental part represented by the point spread function (in the far field) of the ultrasonic apparatus and a moving tissue component described by a collection of scatterers. By computing the convolution of these terms and then the envelope, one obtains a simulated ultrasonic speckle pattern sequence which shows speckle motions closely linked to the tissue dynamics when small motion amplitudes are involved. Here, a theoretical study of the correlation between various linear transformations of the tissue and the corresponding ultrasonic speckle motions is performed, based on a 2D extension of the envelope cross-correlation analysis of a narrow-band Gaussian noise. In the linear scan case, obviously, tissue translation generates an identical speckle translation. However, tissue/speckle motion correlation decreases with increasing rotation and/or biaxial deformation, lateral deformation (perpendicular to the beam propagation axis) being much less sensitive. With respect to the transducer frequency, the rotation and the axial deformation of the tissue show a better relationship with their respective speckle motion at lower frequencies while lateral deformation correlation is independent of the pulse frequency. With respect to beam (pulse) size parameters, tissue/speckle correlation decreases with rotation when a wide ultrasonic beam is used while the axial deformation correlation decreases with the axial duration of the pulse. This study sets the ground for the development of an ultrasonic strain gauge particularly useful for the assessment of biomechanical soft tissue and fluid flow properties based on speckle tracking.
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