Young plants of wheat (Triticum aestivum L. cv. Star), which were treated hydroponically with the triazole fungicide epoxiconazole (BAS 480 F) over a period of 8 days, showed a dose-dependent stimulation of the enzyme activities of the two antifungal hydrolases chitinase and B-1,3-glucanase in the shoot tissue. In the root tissue, no significant rise in the enzyme activities was found. As shown by immunoblot analysis and enzyme-linked immunosorbent assay (ELISA) using antisera against tobacco acidic and basic chitinases and l3-1,3-glucanases, the obeserved increase in the activities coincided with an accumulation of enzyme proteins. This possibly indicates the induction of a de novo synthesis of chitinases and O-1,3-glucanases by epoxiconazole. To our knowledge, this effect of a synthetic fungicide on antifungal hydrolases in an intact plant is demonstrated for the first time.
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