Commercial quartz oscillators of the tuning-fork type with a resonant frequency of ∼ 32 kHz have been investigated in helium liquids. The oscillators are found to have at best Q values in the range 10 5 -10 6 , when measured in vacuum below 1.5 K. However, the variability is large and for very low temperature operation the sensor has to be preselected. We explore their properties in the regime of linear viscous hydrodynamic response in normal and superfluid 3 He and 4 He, by comparing measurements to the hydrodynamic model of the sensor.
Flow due to a commercially available vibrating quartz fork is studied in gaseous helium, He I and He II, over a wide range of temperatures and pressures. On increasing the driving force applied to the fork, the drag changes in character from laminar (characterized by a linear drive vs velocity dependence) to turbulent (characterized by a quadratic drive vs velocity dependence). We characterize this transition by a critical Reynolds number Recrdelta=Ucrdelta/nu, where Ucr is the critical velocity, nu stands for the kinematic viscosity, delta=sqrt[2nu/omega] is the viscous penetration depth, and omega is the angular frequency of oscillations. We have experimentally verified that the corresponding scaling Ucr proportional, sqrt[nuomega] holds in a classical viscous fluid over two decades of nu.
Mutations, haplotypes, and other polymorphic markers in the phenylalanine hydroxylase (PAH) gene were analysed in 133 unrelated Czech families with classical phenylketonuria (PKU). Almost 95% of all mutant alleles were identified, using a combination of PCR and restriction analysis, denaturing gradient gel electrophoresis (DGGE), and sequencing. A total of 30 different mutations, 16 various RFLP/VNTR haplotypes, and four polymorphisms were detected on 266 independent mutant chromosomes. The most common molecular defect observed in the Czech population was R408W (54.9%). Each of the other 29 mutations was present in no more than 5% of alleles and 13 mutations were found in only one PKU allele each (0.4%). Four novel mutations G239A, R270fsdelSbp, A342P, and IVSlnt-8g--a-a were identified. In 14 (5.1%) alleles, linked to four different RFLP/VNTR haplotypes, the sequence alterations still remain unknown. Our results confirm that PKU is a heterogeneous disorder at the molecular level. Since there is evidence for the gene flow coming from northern, western, and southern parts of Europe into our Slavic population, it is clear that human migration has been the most important factor in the spread of PKU alleles in Europe.
We report on the haplotype analysis with polymorphic repeat markers DXS548 and FRAXAC1 next to the FMR1 gene in 37 unrelated fragile X and 36 control chromosomes from Bohemia and Moravia. Our results suggest a significant linkage disequilibrium between fragile X mutations and certain DXS548-FRAXAC1 haplotypes. Allele frequencies obtained differ slightly from those of other European populations with allele 194 being less frequent in our control sample. Rare DXS548 alleles 6.5 (195) and 0 (208) were also present.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.