Large-scale culvert replacement programs could benefit migratory fish populations by reconnecting reproductive and foraging habitats in fragmented watersheds. The objectives of this study were to: (1) identify stream and culvert characteristics contributing to fish passage barriers within an Appalachian watershed, U.S.A.; (2) quantify the total amount of Brook trout (Salvelinus fontinalis) reproductive habitat isolated above culverts; and (3) use an ecological currency to identify culvert replacement priorities and stream mitigation credit opportunities. We surveyed 120 state-owned culverts and used a fish passage assessment filter to determine the ''passability'' of each culvert. We then constructed a geographic information system stream network model to quantify the amount of trout reproductive habitat isolated by culverts. Ninetyseven percent of surveyed culverts were classified as obstacles or complete barriers to trout dispersal. Culvert impassability was higher in small streams with slopes exceeding 3-5%, suggesting a direct relationship between slope and impassability. Thirty-three percent of Brook trout reproductive habitat, representing over 200 km of stream, was isolated by culverts. This is a conservative estimate, because we did not survey privately or federally owned culverts. The top 20 prioritized culverts accounted for nearly half of the habitat loss. Our results indicate that standard culvert designs placed in streams with slopes exceeding 5% consistently produce trout dispersal barriers and should be avoided during new road construction. The process developed here provides an efficient method for identifying culvert replacement priorities and may be used to maximize watershed scale benefits of stream restoration.

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ACKNOWLEDGMENTSThis report was prepared under 3 work assignments of EPA contract #68-C7-0014 to Tetra Tech, Inc. Authors of this report are Jeroen Gerritsen, June Burton, and Michael T. Barbour. We thank Maggie Passmore and Jim Green of EPA Region 3 for helpful guidance, discussions and review. The biological index was made possible by the intensive data collection efforts and discussion of West Virginia DEP; in particular, Janice Smithson, Jeffrey Bailey, Pat Campbell, and John Wirts. This report was prepared with the assistance of Jeffrey White, Erik Leppo, and Brenda Fowler. A Stream Condition Index for West Virginia Wadeable StreamsTetra Tech, Inc. iv March 28, 2000 (Revised July 21, 2000 THIS PAGE LEFT INTENTIONALLY BLANK A Stream Condition Index for West Virginia Wadeable StreamsTetra Tech, Inc. v March 28, 2000 (Revised July 21, 2000 Tetra Tech, Inc. vi March 28, 2000 (Revised July 21, 2000 THIS PAGE INTENTIONALLY LEFT BLANK A Stream Condition Index for West Virginia Wadeable StreamsTetra Tech, Inc. vii March 28, 2000 (Revised July 21, 2000 LIST OF FIGURES Tech, Inc. viii March 28, 2000 (Revised July 21, 2000 LIST OF TABLES EXECUTIVE SUMMARYOver the past century, land use activities such as mining, agriculture, urbanization, and industrialization have seriously threatened the quality of surface waters by contributing to nonpoint-source pollution. In West Virginia, the investigation of these nonpoint sources of water pollution has become a priority. indicator of ecosystem health and can identify impairment with respect to the reference (or natural) condition. The index includes six biological attributes, called metrics, that represent elements of the structure and function of the bottom-dwelling macroinvertebrate assemblage. Metrics are specific measures of diversity, composition, and tolerance to pollution, that include ecological information.The SCI is to be used as the basis for bioassessment in West Virginia and has been calibrated for a long-term biological index period extending from April through October. A data analysis application has been developed to ensure consistency in data management and analysis throughout the state as DEP biologists conduct biological monitoring.Benefits expected from the implementation of the WV SCI will apply to a broad spectrum of management programs, including:characterizing the existence and severity of point and nonpoint source impairment;targeting and prioritizing watersheds and ecosystem management areas for remedial or preventive programs; evaluating the effectiveness of nonpoint source best management programs; screening ecosystems for use attainability; and developing a basis for establishing biocriteria that relate to regional water quality goals, an EPA priority.The West Virginia SCI was tested with independent data collected in 1998 and was able to correctly identify the majority of the stream sites stressed in some way by human disturbance or pollution. Index scores were divided into 5 proposed rating categories for reporting on the condition...

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