We have established a method for quantifying binding of fluorescenc~-labeled growth factors to their receptors on single cells in situ with the confocal laser scanning microscope (CLSM). Biotinylated epidermal growth factor (EGF) coupled to phymrythrin-labeled anti-biotin was used to compare the levels of fluorescence on three different cell types fot which the number of EGF factors was kaown from Scatchard analysis of [12SI]-EGF binding. The results showed that
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