Obesity and type 2 diabetes are strongly associated with abnormal lipid metabolism and accumulation of intramyocellular triacylglycerol, but the underlying cause of these perturbations are yet unknown. Herein, we show that the lipogenic gene, stearoyl-CoA desaturase 1 (SCD1), is robustly up-regulated in skeletal muscle from extremely obese humans. High expression and activity of SCD1, an enzyme that catalyzes the synthesis of monounsaturated fatty acids, corresponded with low rates of fatty acid oxidation, increased triacylglycerol synthesis and increased monounsaturation of muscle lipids. Elevated SCD1 expression and abnormal lipid partitioning were retained in primary skeletal myocytes derived from obese compared to lean donors, implying that these traits might be driven by epigenetic and/or heritable mechanisms. Overexpression of human SCD1 in myotubes from lean subjects was sufficient to mimic the obese phenotype. These results suggest that elevated expression of SCD1 in skeletal muscle contributes to abnormal lipid metabolism and progression of obesity.

High-Intensity Functional Training (HIFT) is a novel exercise intervention that may test body systems in a balanced and integrated fashion by challenging individuals’ abilities to complete mechanical work. However, research has not previously determined if physical work capacity is unique to traditional physiologic measures of fitness. Twenty-five healthy men and women completed a six-week HIFT intervention with physical work capacity and various physiologic measures of fitness assessed pre- and post-intervention. At baseline, these physiologic measures of fitness (e.g., aerobic capacity) were significantly associated with physical work capacity and this relationship was even stronger at post-intervention assessment. Further, there were significant improvements across these physiologic measures in response to the delivered intervention. However, the change in these physiologic measures failed to predict the change in physical work capacity induced via HIFT. These findings point to the potential utility of HIFT as a unique challenge to individuals’ physiology beyond traditional resistance or aerobic training. Elucidating the translational impact of increasing work capacity via HIFT may be of great interest to health and fitness practitioners ranging from strength/conditioning coaches to physical therapists.

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The session rate of perceived exertion method (sRPE) has often been utilized in sports activities in which quantification of external training loads is challenging. The multi-modal, constantly varied nature of high intensity functional training (HIFT) represents a significant hurdle to calculate external work and the sRPE method may provide an elegant solution to this problem. However, no studies have investigated the psychometric properties of sRPE within HIFT interventions. Twenty-five healthy men and women participated in six weeks of HIFT. Rate of perceived exertion and heart rate were assessed within every training session throughout the duration of the intervention. Compared to criterion heart rate-based measures, we observed sRPE method is a valid tool across individual, group, and sex levels. However, poor reliability in participants’ abilities to correctly match rate of perceived exertion with the relative level of physiologic effort (i.e., percentile of maximum heart rate) currently limits the utility of this strategy within HIFT. When applied, the validity and reliability of the sRPE seem to improve over time, and future research should continue to explore the potential of this monitoring strategy within HIFT interventions.

Our study suggests poor success in the acquisition of essential information. Given considerable time and resources, weight loss studies require improved reporting standards to minimize the relatively unsuccessful attempt to contact authors for important and necessary information.