Biofilm formation in a dairy waste irrigation system can reduce treatment capacity and increase maintenance and cleaning. An extreme biofilm observed in a primary treated dairy wastewater system blocked the irrigation line requiring manual cleaning. Both next-generation genomic sequencing and the culturable fraction showed the presence of predominantly Gram-negative bacteria. Isolates identified from current samples and stored samples from the extreme biofilm included Pseudomonas, Citrobacter, Klebsiella and Enterobacter, one Gram-positive spore former (Bacillus cereus) and one unique isolate from the biofilm, a member of the Raoultella genus. Raoultella spp was only cultured from the extreme biofilm; however, next-generation sequencing analysis of a wastewater sample showed this bacterium to be present in the wastewater system. The dominance of Gram negative may be due to the wastewater from the washing equipment used to handle raw milk. Six bacteria from the fresh samples were found to be strong biofilm formers along with the Raoultella spp from the extreme biofilm. Tests using multiple isolates showed Raoultella spp to be important in biofilm formation. This is the first report of the microbial composition of a dairy wastewater biofilm giving insight into the population and growth of microorganisms in the sections of a dairy wastewater irrigation system.
Biofilm formation in dairy wastewater system irrigation pipes can reduce treatment capacity, increasing maintenance and cleaning costs. Understanding the effect of different components in the wastewater on growth and yield of bacteria present could help prevent excessive build-up of biofilms. This study investigated, in aerobic and anaerobic conditions, the effect of calcium, sodium and magnesium concentrations on growth rates, yields and saturation constants of four known biofilm forming bacteria associated with the blockage of an irrigation system. The ions tested (Ca2+, Mg2+, Na+) varied growth rates with biofilm growth in the presence of calcium being significantly slower (P < 0.05) than planktonic growth in sodium. Along with the slower growth, the addition of Ca2+ (up to 0.1 M) increased biofilm formation while addition over 0.5 M prevented biofilm formation. Knowing the nutritional requirements of the bacteria and the effects of the ions will be useful in predicting the growth, development and strategies in controlling biofilm formation in dairy wastewater.
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