Proteins persist longer in the fossil record than DNA, but the longevity, survival mechanisms and substrates remain contested. Here, we demonstrate the role of mineral binding in preserving the protein sequence in ostrich (Struthionidae) eggshell, including from the palaeontological sites of Laetoli (3.8 Ma) and Olduvai Gorge (1.3 Ma) in Tanzania. By tracking protein diagenesis back in time we find consistent patterns of preservation, demonstrating authenticity of the surviving sequences. Molecular dynamics simulations of struthiocalcin-1 and -2, the dominant proteins within the eggshell, reveal that distinct domains bind to the mineral surface. It is the domain with the strongest calculated binding energy to the calcite surface that is selectively preserved. Thermal age calculations demonstrate that the Laetoli and Olduvai peptides are 50 times older than any previously authenticated sequence (equivalent to ~16 Ma at a constant 10°C).DOI: http://dx.doi.org/10.7554/eLife.17092.001

The ability to control fire was a crucial turning point in human evolution, but the question when hominins first developed this ability still remains. Here we show that micromorphological and Fourier transform infrared microspectroscopy (mFTIR) analyses of intact sediments at the site of Wonderwerk Cave, Northern Cape province, South Africa, provide unambiguous evidence-in the form of burned bone and ashed plant remains-that burning took place in the cave during the early Acheulean occupation, approximately 1.0 Ma. To the best of our knowledge, this is the earliest secure evidence for burning in an archaeological context. micromorphology | cooking hypothesis | Homo erectus

Hafting stone points to spears was an important advance in weaponry for early humans. Multiple lines of evidence indicate that ~500,000-year-old stone points from the archaeological site of Kathu Pan 1 (KP1), South Africa, functioned as spear tips. KP1 points exhibit fracture types diagnostic of impact. Modification near the base of some points is consistent with hafting. Experimental and metric data indicate that the points could function well as spear tips. Shape analysis demonstrates that the smaller retouched points are as symmetrical as larger retouched points, which fits expectations for spear tips. The distribution of edge damage is similar to that in an experimental sample of spear tips and is inconsistent with expectations for cutting or scraping tools. Thus, early humans were manufacturing hafted multicomponent tools ~200,000 years earlier than previously thought.

a b s t r a c tThe Fauresmith lithic industry of South Africa has been described as transitional between the Earlier and Middle Stone Age. However, radiometric ages for this industry are inadequate. Here we present a minimum OSL age of 464 AE 47 kyr and a combined U-series-ESR age of 542 À107 þ140 kyr for an in situ Fauresmith assemblage, and three OSL ages for overlying Middle and Later Stone Age strata, from the site of Kathu Pan 1 (Northern Cape Province, South Africa). These ages are discussed in relation to the available lithostratigraphy, faunal and lithic assemblages from this site. The results indicate that the Kathu Pan 1 Fauresmith assemblage predates transitional industries from other parts of Africa e.g. Sangoan, as well as the end of the Acheulean in southern Africa. The presence of blades, in the dated Fauresmith assemblages from Kathu Pan 1 generally considered a feature of modern human behaviour (McBrearty and Brooks, 2000, The revolution that wasn't: a new interpretation of the origin of modern human behavior, J. Human Evolution 39, 453-563),-provides evidence supporting the position that blade production in southern Africa predated the Middle Stone Age and the advent of modern Homo sapiens.