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Abstract. In addition to photosynthesis as in the leaf, fruit possess a system which refixes CO2 from the mitochondrial respiration of predominantly imported carbon. This pathway produces malate by the action of phosphoenolpyruvate carboxylase, PEPC, (E.C. 4.1.1.31) and appears to be regulated primarily by the cytosolic concentration of HCO3/CO2 and malate. Malate is stored in the vacuole as malic acid, constituting a major carbon pool and a potential substrate for respiration. The PEPC in apple fruit proves to be an efficient form of the enzyme with low Michaelis constants, i.e. Km = 0.09 mol m‐3 PEP and 0.2 mol m–3 HCO3, and large Ki= 110 mol m‐3 HCO3. In fleshy fruit, chlorophyll and chloroplasts are unevenly distributed; they resemble the C3 sun‐type and arc concentrated in the perivascular tissue, with smaller chloroplasts, fewer grana per chloroplast and a larger degree of vacuolation than commonly found in a leaf of the same species. Fruit photosynthesis often compensates for respiratory CO2 loss in the light. However, due to respiration in the dark, CO2 loss is in excess of photosynthetic gain in the light, such that a continual loss of CO2 was observed in the diurnal cycle and which is maintained throughout fruit development. The rate of CO2 exchange decreases on a fresh weight or surface basis, but increases with fruit ontogeny on a per fruit basis, causing accumulation of several percent CO2 in the internal cavity. Stomata are present in the outer epidermis of those fruits examined, but with a 10‐to 100‐fold lesser frequency than in the abaxial epidermis of leaf of the same species. The number of Stomata is set at anthesis and remained constant, while the stomatal frequency decreases as the fruit surface expands. Stomata are as sensitive as in leaves in the early stages of fruit development, but often are transformed into lenticels during fruit ontogeny, thereby decreasing the permeability of the outer epidermis. The discrepancy between the CO2‐concentrating mechanism provided by PEPC analogous to C4/CAM Photosynthesis and the kinetics of fruit PEPC, characteristic of C3/non‐autotrophic tissue, suggests the definition of a new type of ‘fruit photosynthesis’ rather than its categorization within an existing type.

Most trees from temperate climates require the accumulation of winter chill and subsequent heat during their dormant phase to resume growth and initiate flowering in the following spring. Global warming could reduce chill and hence hamper the cultivation of high-chill species such as cherries. Yet determining chilling and heat requirements requires large-scale controlled-forcing experiments, and estimates are thus often unavailable. Where long-term phenology datasets exist, partial least squares (PLS) regression can be used as an alternative, to determine climatic requirements statistically. Bloom dates of cherry cv. ‘Schneiders späte Knorpelkirsche’ trees in Klein-Altendorf, Germany, from 24 growing seasons were correlated with 11-day running means of daily mean temperature. Based on the output of the PLS regression, five candidate chilling periods ranging in length from 17 to 102 days, and one forcing phase of 66 days were delineated. Among three common chill models used to quantify chill, the Dynamic Model showed the lowest variation in chill, indicating that it may be more accurate than the Utah and Chilling Hours Models. Based on the longest candidate chilling phase with the earliest starting date, cv. ‘Schneiders späte Knorpelkirsche’ cherries at Bonn exhibited a chilling requirement of 68.6 ± 5.7 chill portions (or 1,375 ± 178 chilling hours or 1,410 ± 238 Utah chill units) and a heat requirement of 3,473 ± 1,236 growing degree hours. Closer investigation of the distinct chilling phases detected by PLS regression could contribute to our understanding of dormancy processes and thus help fruit and nut growers identify suitable tree cultivars for a future in which static climatic conditions can no longer be assumed. All procedures used in this study were bundled in an R package (‘chillR’) and are provided as Supplementary materials. The procedure was also applied to leaf emergence dates of walnut (cv. ‘Payne’) at Davis, California.Electronic supplementary materialThe online version of this article (doi:10.1007/s00484-012-0594-y) contains supplementary material, which is available to authorized users.

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This commentary compares the primary energy requirement for apples (cultivar 'Braeburn'), which were either imported or locally-grown in Meckenheim, Germany. Imported apples of the same cultivar were grown in a Southern hemisphere winter in Nelson, Southland, New Zealand, and were picked at the end of March with subsequent 28 d transport by sea for sale in April in Germany. Locally-grown apples (cultivar 'Braeburn') were picked in mid-October and required a primary energy of nearly 6 MJ/kg of fruit including 0.8 MJoule/kg for five months CA storage at 1 degrees C during a Northern hemisphere winter until mid-March. This compared favourably with 7.5 MJoule/kg for overseas shipment from New Zealand, i.e. a ca. 27% greater energy requirement for these imported fruits. Overall, the primary energy requirement of regional produce, stored several months on-site, partially compensated for the larger energy required to import fresh fruit from overseas. This result is in marked contrast to reported overestimates of a reported up to 8-fold energy requirement for domestic versus imported apple juice concentrate. Our own findings of less primary energy required for domestic apple fruit is discussed with respect to providing local employment, fruit orchards preserving the countryside, quality assurance systems for local fruit such as QS and EUREP-GAP, networking and other factors favouring regional production.