Many Pseudomonas species produce antimicrobial metabolites for pathogen suppression and stimulate the plant growth. So twenty fluorescent pseudomonas strains showed highest antagonism efficiency to soilborne phytopathogens which used throughout the present investigation. These bacteria included five strains P. putida, 3/ P. plecoglossicida, 3/ P. palleroniana, 2/ P. corrugate, 2/ P. entomophila and one from each of P. moraviensis, P. parafulva, P. mosselii, P. anguilliseptica, P. argentinensis. Detection of the genes that encode for the production of antibiotics by these strains such as phenazine-1-carboxylic acid (PCA), phenazine-1-carboxamide (PCN), pyrrolnitrin (PRN), and pyoluteorin (PLT) was done by polymerase chain reaction (PCR) using gene-specific primers. Results clearly showed that eight Pseudomonas strains produced (PCA), twelve strains produced (PCN), nine strains produced (PLT) and two strains produced (PRN). Polymerase chain reaction (PCR) confirmed the obtained result by using TLC. P. putida (SAW19) gave the highest antagonistic potential against tested phytopathogenic fungi and formulated using different carriers to test for its viability on different carriers. The populations of P. putida were markedly higher in vermiculite compared with the carriers under six months incubation period.
Fluorescent Pseudomonads have been effectively utilized for controlling major fungal plant diseases because of their antifungal metabolites. In this study, thirty-four bacterial isolates were isolated from root samples of different crops and screened as potential biological control agents against Rhizoctoniasolani AG 2-2, Pythium arrhenomanes and Fusarium solani. On the basis of dual culture assays, twenty isolates were selected for phenotypic characterization, identification and plant growth promoting traits; four isolates were selected for greenhouse studies. The selected Pseudomonas strains were characterized by PCR methods using rpob primer. Eight showed the ability to produce indole acetic acid (IAA) while 12 isolates have no IAA produced, thirteen isolates could solubilize inorganic phosphate and six strains produced hydrogen cyanide. Tested Pseudomonas strains had positive response on bean growth and health.Obtained results indicated that the tested antagonistic fluorescent pseudomonads reduced disease severity in beans and disease development under greenhouse pot experiment. However, further study is needed to apply to these results under field conditions.
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