Objectives: Increased generation of free radicals exceeding the antioxidant capacity of the host is deleterious. Thus the search for new, potent and safe antioxidants will be a valuable addition to the limited antioxidant arsenals available. Therefore, the antioxidant activity, cytotoxicity potential and phytochemical constituents of 70% methanol extract of the seed of Spondias mombin (MESSM) was investigated. Material and Methods: Antioxidant activity of MESSM was evaluated in 1-Diphenyl-2picrylhydrazyl (DPPH), hydrogen peroxide and nitric oxide assays. Cytotoxicity of the extract was evaluated against rhabdomyosarcoma (RD) cell line in a MTT based assay. Phytochemical composition of MESSM was determined using the Gas Chromatography-Mass Spectrometry (GC-MS). Results: MESSM produced better antioxidant activity in DPPH (IC 50 = 58.64 ± 1.49 µg/mL) and H2O2 (IC50 = 44.03 ± 5.57 µg/mL) than in the NO (IC50 = 494.55 ± 12.68 µg/mL, p<0.0001) assays. Also, MESSM was non-toxic (CC 50 = 139.6 ± 0.54 µg/mL) in comparison to cyclophosphamide (CC50 = 0.97 ± 0.03 µg/mL) against rhabdomyosarcoma (RD) cell line. The major compounds in MESSM were dodecanoic acid (22.48%), tetradecanoic acid (17.95%), n-hexadecanoic acid (15.35 %), Capsaicin (12.11%) and dihydrocapsaicin (5.23 %). Conclusion: The seed extract of Spondias mombin lacked cytotoxicity potential. It contains non-toxic antioxidant compounds that could be explored in pharmaceutical and cosmetics industries for development of antioxidant agents.
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