This study aimed to determine the methicillin resistance and some genotypic characteristics of staphylococci in dogs and their owners. A total of 132 swab samples from 33 healthy dogs and their owners were taken. Isolated staphylococci were identified by PCR. The antibiotic sensitivities of the isolates were determined by disc diffusion method. Determination of pvl, mecA, bla, and fem genes and SCCmec types was performed by PCR. Isolates were genotyped according to coa and spa gene polymorphisms by PCR. Fortyfive isolates were identified as Stapyloccoccus spp. Among them, 8 isolates were identified as S. aureus and 23 isolates were identified as S. pseudintermedius. According to the disc diffusion tests, methicillin resistance methicillin-resistant was determined in all S. aureus (MRSA). Among the methicillin-resistant strains, 8 were S. pseudintermedius (MRSP). However, methicillin resistance was determined genotypically in 10 strains and of these 5 were MRSA and 2 were MRSP. Two S. aureus strains and 7 S. pseudintermedius strains were mec A negative but bla positive. No strain carried the fem gene. Ten different coa types were detected among the strains. All MRSA strains carried pvl genes. In conclusion, MRSP should be considered to pose a risk for humans living with dogs or in contact with them.

In this study, it was aimed to develop an ELISA diagnostic kit that can be used in the serological investigation of Q fever disease caused by C. burnetii. For this purpose, bovine, sheep, goat and buffalo sera were tested with a commercial ELISA kit and home-made ELISA kit developed using two different conjugates. Of the 92 buffaloes blood serum samples tested, 22 (23.9%) were found positive by commercial ELISA kit, 7 (7.6%) and 29 (31.5%) were positive by protein-A conjugated ELISA and 29 anti-bovine IgG conjugated ELISA, respectively. In addition, Q fever positive and negative cattle, sheep, and goat blood sera that were found to be positive and negative for Q fever in a previous study conducted on animals with abortion history were also re-examined in our laboratory. In conclusion, within the scope of the project, an alternative homemade ELISA kit was developed for the serological diagnosis of Q fever and serum samples of the target species of buffalo, cattle, sheep, and goats were tested. Thus, the home-made ELISA kit, which can be used in research aimed at detecting seropositivity, especially in buffalo sera, was made ready for use. It was concluded that the data obtained would contribute to wider ranging epidemiological studies. The epidemiology of the disease can be revealed in detail in the light of research to be carried out with more comprehensive projects in our region.

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Öz: Mandaların diğer hayvanlarda olduğu gibi bazı hastalıkların duyarlı hayvan popülasyonlarına ve insanlara bulaştırılmasında rol oynadıkları ortaya konulmuştur. Bu çalışmada Amasya ili ve ilçelerinde yetiştiriciliği yapılmakta olan Anadolu Mandalarından toplanan 140 dışkı örneği termofilik Campylobacter türleri açısından incelendi. Dışkı örneklerinden termofilik Campylobacter türlerinin izolasyonu amacıyla standart selektif zenginleştirme tekniği kullanıldı. Campylobacter şüpheli izolatların cins ve tür düzeyinde identifikasyonları PCR ile yapıldı. 140 dışkı örneğinden 13 (% 9,3) termofilik Campylobacter cins düzeyinde identifiye edildi. İdentifiye edilen 13 izolatın 2 (% 15,4)'si C. jejuni, 1 (% 7,7)'i C. coli olarak isimlendirilirken, geriye kalan 10 izolat Campylobacter spp. olarak tanımlandı. C. jejuni izolatlarında ctx (cytolethal distenting toxin) genlerinin (cdtA, cdtB ve cdtC) tespiti mPCR ile gerçekleştirildi. İzolatların hiç birinde cdt geni saptanamadı. Bu araştırma ile bölgemizde ilk kez Anadolu Mandalarından sağlanan dışkı örnekleri termofilik Campylobacter türleri yönünden incelendi. Araştırmadan elde edilen verilerin, yöremizde yapılacak benzer çalışmalara kaynak teşkil edebileceği kanısına varıldı.

Pseudomonas aeruginosa is frequently isolated from various disease and nosocomial infections. It is clinically important because of its multiple antibiotic resistance and biofilm production. The aims of this study were to determine the production of biofilm and to make antibiotyping and genotyping of P. aeruginosa strains isolated from bronchial aspirate samples of dogs with pneumonia in Turkey and Romania. For this purpose, biofilm properties of 10 Turkish and 10 Romanian isolates were determined by Congo red agar method. The resistance profiles of the isolates were determined by Kirby-Bauer Disk Diffusion Method. The antibiotyping was performed according to the resistance profiles of the isolates. Genotypical similarities were determined by RAPD-PCR. In terms of biofilm production, all of the isolates examined in this study were found to be positive. In consequence of the antibiograms, the resistance rates against enrofloxacin, ceftriaxone, amoxicillin + clavulanic acid, ciprofloxacin, meropenem, colistin, gentamicin and azithromycin were stated as 30%, 70%, 100%, 40%, 30%, 10%, 10%, 100% for Turkish isolates and 30%, 60%, 100%, 30%, 10%, 0%, 10%, 100% for Romanian isolates, respectively. As a result of antibiotyping, similarities of the isolates from Turkey and Romania were determined to be between 86-100%. Phylogenotypical similarities of the isolates from Turkey and Romania were determined to be between 47-96%. It was concluded that antibiotic resistances of isolates were high and resistance against antibiotics used in the treatment could be formed due to biofilm production.

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Pododermatitis is defined as the inflammation of the paw skin of nails, interdigital space, base pads, and nail folds. Complex conditions known as pedal folliculitis and furunculosis often need complicated diagnosis and treatment because of their multifactorial nature. Four dogs with symptoms of chronic and progressive pododermatitis were admitted to the Ondokuzmayis University Animal Hospital. Bacteriological examination of exudates revealed Methicillin Resistant Staphylococcus aureus (MRSA) infection in German Shepherd, Labrador Retriever and Setter dogs. The MRSA isolate was sensitive only to teicoplanin, gentamicin and enrofloxacin, respectively. From the infection of the mixed-breed dog, ampicillin/sulbactam susceptible Vancomycin-Resistant Enterococcus spp. was isolated. Antinuclear Antibody Tests revealed high positive titers. The use of antibiotics sensitive for each dog together with the combination of prednisolone treatment resulted in significant recovery. In conclusion, antibiotic-resistant bacteria and immunomodulatory responsive etiology should be considered together in cases of chronic pododermatitis in dogs.