Tissue culture techniques in tomato, pepper and eggplant are important for the development of disease-resistant and high yielding varieties, which require a suitable regeneration protocol. Although shoot regeneration has been achieved by using different explants and cytokinin doses in Solanaceae species, very few studies have reported in vitro regeneration using root tissues. The current study is the first report to compare direct shoot regeneration capabilities using root node explants in three Solanaceae species (tomato, pepper, and eggplant) under three cytokinins (BAP, TDZ, and GA3) hormone. Plantlets were regenerated from the root node explants of tomato, pepper and eggplant in the media containing 0, 0.5, 1, 1.5 and 2mg/L of BAP, TDZ and GA3. Results revealed that the shoot regeneration of root node explants varied according to the species, cytokinins (BAP, TDZ and GA3) and doses of hormones. Among the species, the best shoot regeneration was observed in tomato followed by eggplant and pepper plants. While the shoot length was statistically significant in tomato, it was observed to be insignificant in pepper and eggplant. The highest number of root regeneration and root length was observed in tomatoes. The results obtained from the study will contribute to the development of successful/reproducible tissue culture protocols from roots node explants.
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.
Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc.) obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as ‘Partially Incompatible’ and 12 genotypes involving 4 S-alleles were assigned ‘Totally Incompatible’. No S-alleles were observed in 2 genotypes (Pancarlık and Hüryemez) which exhibited ‘Compatibility’ status.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.