Objectives To determine of antioxidant, cytotoxic and tyrosinase inhibitory (TI) activities, and phenolic profiles with a new high performance liquid chromatography (HPLC) method of green, white and black teas. Methods Antioxidant activities of the teas were examined by means of scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC). The phenolic contents were investigated by means of HPLC. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the cytotoxic potential of tea extracts in cancer and normal cell lines. TI activity was investigated against diphenolase (DOPA), using kojic acid as a positive control. Results The green tea extract exhibited stronger antioxidant than white and black tea extracts. The green tea contained syringaldehyde, p-coumaric acid, benzoic acid, and quercetin. The white tea extract had the highest tyrosinase inhibitor activity. The extracts exhibited higher cytotoxic potential toward cancer cells than normal cells. The methanol extract of green tea had the highest cytotoxic potential, while the water extracts were less toxic. Conclusion The green, white, and black teas can be regarded as an important in terms of rich antioxidant, tyrosinase inhibitor, and cytotoxic activities in both the pharmaceutical and food industries.
The objective of present study was to specify the antioxidant, antimicrobial, cytotoxic, anticholinesterase, and antityrosinase activities of Verbascum oocarpum Murb., together with its volatile components. The methanolic and aqueous extracts of aerial parts of V. oocarpum indicated substantial antioxidant activity derived from benzoic acid, sinapic acid, and quercetin compounds. The methanolic extract displayed moderate antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, and Mycobacterium smegmatis. It also exhibited a higher IC 50 value on tyrosinase than kojic acid, and lower acetylcholinesterase and butyrylcholinesterase inhibitor activities than galantamin. MTT analysis revealed that high concentrations of V. oocarpum extract can result in cytotoxicity, with an IC 50 of 0.444 mg/mL. Common volatile components included pentadecane, hex-2(E)-enal, limonene, phenylacetaldehyde, isophorone, 1-methoxy-4-(2propenyl)-benzene, 5,6,7,7a-tetrahydro-4,4,7a-trimethyl-2(4h)-benzofuranone and hexadecane. V. oocarpummay be of potential benefit to the food, cosmetical, and pharmaceutical sectors owing to its potent anticholinesterase, antioxidant, and antimicrobial activities, volatile components, and limited cytotoxicity.
The goal of our study was to examine of antioxidant, antimicrobial, anticholinesterase activities, and phenolic composition of Psephellus huber-morathii. The antioxidant activities of extracts have been assessed by Ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging. Phenolic constituents were measured using reverse phase-high performance liquid chromatography (RP-HPLC), and Korkmaz et al.; JPRI, 26(1): 1-10, 2019; Article no.JPRI.47659 2 antimicrobial activity was investigated using the agar well diffusion method. Total phenolic content, FRAP, and CUPRAC results of aqueous extract have been better than methanolic extract, except for DPPH activity. Benzoic acid, and p-coumaric acid as major phenolic compounds were specified. Methanolic extract was especially effective against all microorganisms tested except for Yersinia pseudotuberculosis. The methanolic extract have been displayed inhibitory effect on tyrosinase. All extracts have been exhibited lower acetylcholinesterase, and butyrylcholinesterase inhibitory activities than galantamine. P. huber-morathii can be considered in the food, and drug industries due to antioxidant capacity and antimicrobial activities of the species. It can be potential source as anti-browning agents because of its average tyrosinase inhibitory activity. Original Research Article
In the present study, ethnomedicinal uses of the plants naturally growing in 35 villages of Akkuş District have been determined. Informations about the uses of the plants have been obtained from villagers using a questionnaire. At the end of the identification of the plants collected from villages, 58 taxa belong to 32 families have been determined. Furthermore, tyrosinase enzyme activity studies of methanolic extracts of all the species carried out spectroscopicallly. Eventually, the plants has been demonstrated to be used as folk medicine, food animal feed, ornamental, stuff, dyeing, odour, insecticide and fishing. According to the biological screening studies, some species have been indicated to have inhibitory effect, but the others have activator effect on tyrosinase enzyme.
The aim of the present study is to determine the potent biological activities and carry out isolation studies on Barbarea integrifolia. The antioxidant capacity of the species was evaluated by total phenolic content, FRAP, CUPRAC, and DPPH radical scavenging activity. Anticancer activity studies were performed by MTT assay in MDA-MB-231, MCF-7, Hep3B, PC-3, A549, HCT116, L-929 cell lines. It was observed that the remaining aqueous fraction has higher total phenolic content while higher activity in the CUPRAC and FRAP assays was displayed for the methanolic extract and chloroform fraction. The extracts showed anticancer activity as compared with vincristine. It was observed that chloroform fraction has the highest anticancer activity on MCF-7 cell line, while ethyl acetate fraction has the highest anticancer activity on Hep-3B and A549 cell lines. Methanolic extract has the highest anticancer activity on HCT116 and MDA-MB-23 cell lines. The isolation studies have been performed using several chromatographic methods. The chemical structures of compounds have been identified by means of 1H NMR, 13C NMR, 2D-NMR, and MS. Five major compounds, one steroid (β-Sitosterol), one phenolic acid (Rosmarinic acid), one flavonol heteroside (kaempferol 7-O-α-l-rhamnoside-3-O-β-d-(2-O-β- d -glucosyl)-β-d-glucoside), and two glucosinolates (Gluconasturtiin, Gluconasturtiin choline salt) have been isolated.
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