Most children, in the age of nursery consume food that contains both colorants and preservatives with great amount. This observation led us to study the interaction between sodium nitrite as a food preservative and sun set yellow as a coloring agent. The mixture of the two agents at the limited dose of each was found to be a lethal dose. So, 1/10 th of this dose was used daily for 30 days. Animals were divided into three groups. The first group served as a control, while the second group was orally administered a mixture of 10 mg sod.nitrite (NaNO3)/kg mixed with 0.5 mg/kg/day sun set yellow (S.S.Y). The third group received garlic (5 mcg/kg) in addition to the above mentioned mixture. After 30 days of treatment, half of the animals from each group were decapitated. The other half of the animals was left for another 15 days without any additional treatment as a recovery period.Ingestion of the mixture of (NaNO3 and S.S.Y) significantly decreased rat body weight, RBCs and WBCs counts, Hb%, Hct%. No changes were recorded for organ/ body weight, respiratory rate, heart rate, rectal temperature.A complete recovery from the abnormalities of most physiological and hematological parameters was observed after the recovery period or when garlic was administered.
LOVAZA®, containing purified fish oil (FO), is widely prescribed for the treatment of hyperlipidemia, however its efficacy has never been tested in Systemic Lupus Erythematosus (SLE) prone mice (NZB/NZW) F1 (B/W), in comparison to regular FO. We investigated for the first time, a dose dependent effect of LOVAZA®, compared to placebo and regular FO with standard diet as a control, in a well‐established animal model of human SLE. Weanling B/W female mice were divided into 6 groups fed nutritionally adequate semi purified diet supplemented with LOVAZA® (1% and 4%), placebo (1% and 4%), FO (4%) [18/12:eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA)] and standard lab chow ad libitum. Proteinuria and anti‐dsDNA antibodies were measured to assess the disease progression. Further, we monitored survival and evaluated inflammatory cytokines including kidney histology. Results showed that LOVAZA®(4%) significantly extends both the median and maximal lifespan of B/W mice than regular FO (4%), placebo (4%) and standard lab chow diet. Additionally, LOVAZA® (4%) also decreased serum anti‐dsDNA antibodies, reduced proteinuria with age, lowered LPS‐stimulated inflammatory cytokines (TNF‐α and IL‐6) produced by splenocytes and reduced nephritis in SLE condition. These data indicate that LOVAZA® (4%), containing purified FO is found more effective in suppressing inflammation and extending lifespan of SLE‐prone B/W mice. Further studies in SLE patients are needed to confirm this observation.Funding support: 1R01AT004259‐01 from NIH/NCCAMGrant Funding Source: 1R01AT004259‐01 from NIH/NCCAM
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