In this study, the characterization and the antimicrobial properties of nano silver (nAg) coating on leather were investigated. For this purpose, turbidity, viscosity and pH of nAg solutions prepared by the sol-gel method were measured. The formation of films from these solutions was characterized according to temperature by Differential Thermal Analysis-Thermogravimetry (DTA-TG) equipment. The surface morphology of treated leathers was observed using Scanning Electron Microscopy (SEM). The antimicrobial performance of nAg coatings on leather materials to the test microorganisms as Escherichia coli
, Staphylococcus aureus
, Candida albicans
and Aspergillius niger
was evaluated by the application of qualitative (Agar overlay method) and quantitative (percentage of microbial reduction) tests. According to qualitative test results it was found that 20 μg/cm
2
and higher concentrations of nAg on the leather samples were effective against all microorganisms tested. Moreover, quantitative test results showed that leather samples treated with 20 μg/cm
2
of nAg demonstrated the highest antibacterial activity against E. coli
with 99.25% bacterium removal, whereas a 10 μg/cm
2
concentration of nAg on leather was enough to exhibit the excellent percentage reduction against S. aureus
of 99.91%. The results are promising for the use of colloidal nano silver solution on lining leather as antimicrobial coating.
In the present research, the antimicrobial effects of nanosized silver (Ag) doped TiO(2) colloidal solutions prepared using a sol-gel technique were investigated. In order to determine the solution characteristics, the turbidity, viscosity and pH of the colloidal solutions were measured. Differential thermal analysis-thermogravimetry equipment was used to determine the chemical structures and reaction types of the films formed from these solutions. The morphology of Ag doped TiO(2) nanoparticles was evaluated by atomic force microscopy. The disc diffusion method was employed to explore antimicrobial activity, and the Broth Microdilution method was used to obtain MIC values of nanosized Ag doped TiO(2) colloidal solutions against the test microorganisms Escherichia coli, Staphylococcus aureus, Candida albicans, Bacillus subtilis, and Salmonella typhimurium. It was found that the silver doped TiO(2) nanoparticles inhibited the growth and multiplication of the test microorganisms, including the fungus C. albicans. Antimicrobial activity was observed against all tested microorganisms at a very low concentration of 1.125-2.81 μg/ml of nano silver in 1-25 % Ag-TiO(2) solutions.
In this study, silver (Ag) doped hydroxyapatite (HA) was produced by the microwave method and its antimicrobial activity was investigated. The physical, chemical and structural characteristics of the material were determined using multipurpose X-ray diffractometry (XRD), Fourier transform infrared spectrometry (FTIR), and scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDS) apparatus. The amount of silver in the solutions of silverdoped hydroxyapatite obtained were determined with the use of an inductively coupled plasma optical emission spectroscopy instrument (ICP-OES). The minimum inhibition concentration (MIC) of the silver doped hydroxyapatite (Ag-HA) against the test microorganisms was determined by the Broth Microdilution method. It was established that a concentration of 2.09-12.25 µg/ml was effective against gram-negative bacteria (Escherichia coli ATCC 12228, Salmonella typhimurium CCM 5445, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae CCM 2318), and 4.18-12.25 µg/ml was effective against gram-positive bacteria (Staphylococcus aureus ATCC6538-P, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212) and the yeast Candida albicans ATCC 10239.
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