Increasing evidence suggests that diverse activation patterns of metabolic signalling pathways may lead to molecular diversity of cervical cancer (CC). But rare research focuses on the alternation of fatty acid metabolism (FAM) in CC. Therefore, we constructed and compared models based on the expression of FAM-related genes from the Cancer Genome Atlas by different machine learning algorithms. The most reliable model was built with 14 significant genes by LASSO-Cox regression, and the CC cohort was divided into low-/high-risk groups by the median of risk score. Then, a feasible nomogram was established and validated by C-index, calibration curve, net benefit, and decision curve analysis. Furthermore, the hub genes among differential expression genes were identified and the post-transcriptional and translational regulation networks were characterized. Moreover, the somatic mutation and copy number variation landscapes were depicted. Importantly, the specific mutation drivers and signatures of the FAM phenotypes were excavated. As a result, the high-risk samples were featured by activated de novo fatty acid synthesis, epithelial to mesenchymal transition, angiogenesis, and chronic inflammation response, which might be caused by mutations of oncogenic driver genes in RTK/RAS, PI3K, and NOTCH signalling pathways. Besides the hyperactivity of cytidine deaminase and deficiency of mismatch repair, the mutations of POLE might be partially responsible for the mutations in the high-risk group. Next, the antigenome including the neoantigen and cancer germline antigens was estimated. The decreasing expression of a series of cancer germline antigens was identified to be related to reduction of CD8 T cell infiltration in the high-risk group. Then, the comprehensive evaluation of connotations between the tumour microenvironment and FAM phenotypes demonstrated that the increasing risk score was related to the suppressive immune microenvironment. Finally, the prediction of therapy targets revealed that the patients with high risk might be sensitive to the RAF inhibitor AZ628. Our findings provide a novel insight for personalized treatment in CC.
Planting ratoon rice can realize one sowing and two harvests, which is of great significance for improving grain yield. However, the effects of nitrogen (N) regime in the main crop on the grain yield of ratoon rice and the associated physiological mechanisms are not clearly understood. The indica hybrid rice Liangyou 6326 was used, and three N fertilizer levels (100 kg ha−1 (low N, LN), 250 kg ha−1 (medium N, MN), and 400 kg ha−1 (high N, HN)) and four different ratios of basal tillering fertilizer to panicle fertilizer (7:3, 6:4, 5:5, and 4:6) applied to the main crop were designed to investigate their effects on the grain yields of the main and ratoon crops. The results showed that excessive N application rate and panicle N application rate in the main crop was not conducive to the improvement of yield and agronomic nitrogen use efficiency (ANUE) in both seasons. The increased yield in the ratoon crop was attributed to the increase in the regeneration rate. Appropriate increasing of the panicle N application rate was beneficial for increasing the ROA and NSC concentration in the main crop, resulting in an increase in the number, length, and fresh weight of regenerated buds, which caused an improvement in the regeneration rate. However, when excessive panicle N was applied in the main crop, the excessive germination of regenerated buds decreased the length and fresh weight of the regenerated bud and resulted in a decrease in the regeneration rate. These results suggest that in the production of ratoon rice, reasonable N regime in the main crop could increase the yield and ANUE in both seasons.
Alternate wetting and drying irrigation is a widely used irrigation method for water‐savings and high‐yielding in rice (Oryza sativa L.) production; however, its effect on grain yield (GY) in the main and ratoon crops is unclear. With two rice cultivars as materials, the effects on GY in the main and ratoon crop of three irrigation regimes, that is, conventional flooding irrigation (CI), alternate wetting and moderate soil drying irrigation (WMD), and alternate wetting and severe soil drying irrigation (WSD), during grain filling of the main crop were investigated. Compared with CI, WMD increased GY by 6.0∼6.5% in the main crop by increasing the 1,000‐grain weight and filled grain rate, whereas the ratoon crop GY increased by 13.3∼14.6% via increased panicle number. The WSD had no significant effects on the main crop GY, but significantly decreased ratoon crop GY by decreasing panicle number. A higher leaf photosynthetic rate, leaf area index, root oxidation activity, dry matter accumulation and nonstructural carbohydrate (NSC) translocation under WMD primarily drove the increased 1,000‐grain weight and filled grain rate in the main crop. Under WMD, higher soluble sugar contents catalysed by high amylase activity and higher zeatin + zeatin riboside in stems of the main crop promoted the growth of regenerated buds and increased the ratoon crop panicle number. In conclusion, WMD during grain filling of the main crop improved the growth and development of the main crop and promote the germination of regenerated buds, thereby increasing the GY of both main and ratoon crops.
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