A visible light‐initiated pseudo four‐component reaction of salicylaldehydes, indole and malononitrile in aqueous ethyl lactate is described. This pot, atomic and step economic (PASE) methodology provides a practical approach for the preparation of various 5‐substituted indole chromeno[2,3‐b]pyridines from readily available starting materials at ambient temperature without any catalyst. High yields and excellent functional groups tolerance are observed.magnified image

Cells do not live in a vacuum, but in a milieu defined by cell-cell communication that can be measured via emerging high-resolution spatial transcriptomics approaches. However, analytical tools that fully leverage such data for kinetic modeling remain lacking. Here we present Spateo (http://spateo-release.readthedocs.io/), a general framework for quantitative spatiotemporal modeling of single-cell resolution spatial transcriptomics. Spateo delivers novel methods for digitizing spatial layers/columns to identify spatially-polar genes, and develops a comprehensive framework of cell-cell interaction to reveal spatial effects of niche factors and cell type-specific ligand-receptor interactions. Furthermore, Spateo reconstructs 3D models of whole embryos, and performs 3D morphometric analyses. Lastly, Spateo introduces the concept of "morphometric vector field" of cell migrations, and integrates spatial differential geometry to unveil regulatory programs underlying various organogenesis patterns of Drosophila. Thus, Spateo enables the study of the ecology of organs at a molecular level in 3D space, beyond isolated single cells.

Neural network-based model for text-to-speech (TTS) synthesis has made significant progress in recent years. In this paper, we present a cross-lingual, multi-speaker neural end-to-end TTS framework which can model speaker characteristics and synthesize speech in different languages. We implement the model by introducing a separately trained neural speaker embedding network, which can represent the latent structure of different speakers and language pronunciations. We train the speech synthesis network bilingually and prove the possibility of synthesizing Chinese speaker's English speech and vice versa. We explore different methods to fit a new speaker using only a few speech samples. The experimental results show that, with only several minutes of audio from a new speaker, the proposed model can synthesize speech bilingually and acquire decent naturalness and similarity for both languages.

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