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Living trees in forests emit methane (CH 4 ) from their stems. However, the magnitudes, patterns, drivers, origins, and biogeochemical pathways of these emissions remain poorly understood.We measured in situ CH 4 fluxes in poplar stems and soils using static chambers and investigated the microbial communities of heartwood and sapwood by sequencing bacterial 16S, archaeal 16S, and fungal ITS rRNA genes.Methane emissions from poplar stems occurred throughout the sampling period. The mean CH 4 emission rate was 2.7 mg m −2 stem d −1 . Stem CH 4 emission rate increased significantly with air temperature, humidity, soil water content, and soil CH 4 fluxes, but decreased with increasing sampling height. The CO 2 reduction and methylotrophic methanogenesis were the major methanogenic pathways in wood tissues. The dominant methanogen groups detected in stem tissues were Methanobacterium, Methanobrevibacter, Rice Cluster I, Methanosarcina, Methanomassiliicoccus, Methanoculleus, and Methanomethylophilaceae. In addition, three methanotrophic genera were identified in the heartwood and sapwood -Methylocystis, Methylobacterium, and Paracoccus.Overall, stem CH 4 emissions can originate directly from the internal tissues or co-occur from soils and stems. The co-existence of methanogens and methanotrophs within heartwood and sapwood highlights a need for future research in the microbial mechanisms underlying stem CH 4 exchange with the atmosphere.

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In forest ecosystems, the majority of methane (CH4) research focuses on soils, while tree stem CH4 flux and driving factors remain poorly understood. We measured the in situ stem CH4 flux using the static chamber-gas chromatography method at different heights in two poplar (Populus spp.) forests with separate soil textures. We evaluated the relationship between stem CH4 fluxes and environmental factors with linear mixed models and estimated the tree CH4 emission rate at the stand level. Our results showed that poplar stems were a net source of atmospheric CH4. The mean stem CH4 emission rates were 97.51 ± 6.21 μg·m−2·h−1 in Sihong and 67.04 ± 5.64 μg·m−2·h−1 in Dongtai. The stem CH4 emission rate in Sihong with clay loam soils was significantly higher (P < 0.001) than that in Dongtai with sandy loam soils. The stem CH4 emission rate also showed a seasonal variation, minimum in winter and maximum in summer. The stem CH4 emission rate generally decreased with increasing sampling height. While the differences in CH4 emission rates between stem heights were significant in the annual averages, these differences were driven by differences observed in the summer. Stem CH4 emission rates were significantly and positively correlated with air temperature (P < 0.001), relative humidity (P < 0.001), soil water content (P < 0.001), and soil CH4 flux (P < 0.001). At these sites, the soil emitted CH4 to the atmosphere in summer (mainly from June to September) but absorbed CH4 from the atmosphere during the other season. At the stand level, tree CH4 emissions accounted for 2–35.4% of soil CH4 uptake. Overall, tree stem CH4 efflux could be an important component of the forest CH4 budget. Therefore, it is necessary to conduct more in situ monitoring of stem CH4 flux to accurately estimate the CH4 budget in the future.

Improved mechanistic understanding of soil methane (CH4) exchange responses to shifts in soil moisture and temperature in forest ecosystems is pivotal to reducing uncertainty in estimates of the soil-atmospheric CH4 budget under climate change. We investigated the mechanism behind the effects of soil moisture and temperature shifts on soil CH4 fluxes under laboratory conditions. Soils from the Huai River Basin in China, an area that experiences frequent hydrological shifts, were sampled from two consecutive depths (0–20 and 20–50 cm) and incubated for 2 weeks under different combinations of soil moisture and temperature. Soils from both depths showed an increase in soil moisture and temperature-dependent cumulative CH4 fluxes. CH4 production rates incubated in different moisture and temperature in surface soil ranged from 1.27 to 2.18 ng g−1 d−1, and that of subsurface soil ranged from 1.18 to 2.34 ng g−1 d−1. The Q10 range for soil CH4 efflux rates was 1.04–1.37. For surface soils, the relative abundance and diversity of methanotrophs decreased with moisture increase when incubated at 5 °C, while it increased with moisture increase when incubated at 15 and 30 °C. For subsurface soils, the relative abundance and diversity of methanotrophs in all samples decreased with moisture increase. However, there was no significant difference in the diversity of methanogens between the two soil depths, while the relative abundance of methanogens in both depths soils increased with temperature increase when incubated at 150% water-filled pore space (WFPS). Microbial community composition exhibited large variations in post incubation samples except for one treatment based on the surface soils incubated at 15 °C, which showed a decrease in the total and unique species number of methanotrophs with moisture increase. In contrast, the unique species number of methanogens in surface soils increased with moisture increase. The analysis of distance-based redundancy analysis (db-RDA) showed that soil pH, dissolved organic carbon (DOC), dissolved organic nitrogen (DON), microbial biomass carbon (MBC), NO3−-N, and NH4+-N mainly performed a significant effect on methanotrophs community composition when incubated at 60% WFPS, while they performed a significant effect on methanogens community composition when incubated at 150% WFPS. Overall, our findings emphasized the vital function of soil hydrology in triggering CH4 efflux from subtropical plantation forest soils under future climate change.