Here, we present the complete genome sequence and annotation of Ralstonia syzygii subsp. indonesiensis strain LLRS-1, which caused bacterial wilt on flue-cured tobacco in Yunnan province, southwest China. Strain LLRS-1 is the first R. syzygii strain identified to be pathogenic to tobacco in China. The completely assembled genome of strain LLRS-1 consists of a 3648314-bp circular chromosome and a 2046405-bp megaplasmid with 5190 protein-coding genes, 55 tRNAs, 28 sRNAs, 3 structural RNAs ( 5S, 16S, and 23S), and has a G+C content of 67.05%.
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