The present study deals with the optimization of phenolic compounds extraction from artichoke (Cynara scolymus L.) waste, and development of a functional enriched edible oil with potential health promoting effects.. Box-Behnken Design (BBD), a widely used form of response surface methodology (RSM), was applied to investigate the effect of process variables on the microwave-assisted extraction (MAE) for the recovery of total phenolic compounds (TPC). Independent variables including ethanol concentration (%), microwave power (W), irradiation time (min), solvent-to-material ratio (mL/g) for MAE were studied. The statistical analysis revealed that the optimal MAE conditions were obtained with 72 % ethanol as extraction solvent, 81:1 mL/g of solvent/solid ratio, 4 min and 900 W for irradiation time and power, respectively. Maximum predicted TPC recovery under the optimized conditions was 37.43 ± 1.26 mg GAE/g DW, which was close to the experimental value 36.10 ± 0.72, indicating suitability of the employed model and the success of RSM in optimizing the extraction conditions. The TPC confirmed the efficiency of MAE methods when compared with the conventional method. The studied edible oil enriched with the optimized extract had a content of phenolic compounds equivalent to 56.41 ± 2.03 µg/mL compared to the control oil.
The present study aims to optimize the extraction of phenolics by microwave-assisted extraction (MAE) using the response surface methodology (RSM), from Lemon verbena leaves. The optimized extract was tested for its antioxidant activity using two methods (DPPH and reducing power) and its antibacterial efficiency by using disk diffusion assay and broth microdilution, against two Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) and two Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633) strains. Under the optimized conditions (40% (v/v): of ethanol concentration, 188 s of irradiation time, 600 W of microwave power and 1:40 g/mL of solid-to-liquid ratio) the total phenolic content (TPC) was 67.87±1.61 mg GAE/g DW. The IC50 of the extract was 139.65±1.44 µg/mL and 56.60±2.79 µg/mL for DPPH inhibition and reducing power, respectively. The best antibacterial activity was shown by the extract obtained by MAE with lower MBC (1.56 to 18.75 mg/mL) and MBC/MIC ratio. Lemon verbena extract can be used as an ingredient in cosmetics, food supplements and herbal medicinal products due to its interesting biological properties.
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