Abstract. A two-well forced-gradient experiment involving virus and microsphere transport was carried out in a sandy aquifer in Borden, Ontario, Canada. Virus traveled at least a few meters in the experiment, but virus concentrations at observation points 1 and 2.54 rn away from the injection well were a small fraction of those injected. A simplified planar radial advection-dispersion equation with constant dispersivity, coupled with equilibrium and reversible first-order mass transfer, was found to be adequate to simulate the attachment and transport process. During the experiment a short-duration injection of high-pH water was also made, which caused detachment of previously attached viruses. For simulating this detachment and associated transport, the same transport and masstransfer equations were used; but all rate parameters were varied as groundwater p H changed from 7.4 to 8.4 and then back to 7.4. The physicochemical parameters obtained from fitting breakthrough curves at one sampling well were used to predict those at another well downstream. However, laboratory-determined parameters overpredicted colloid removal. The predicted pattern and timing of biocolloid breakthrough was in agreement with observations, though the data showed a more-disperse breakthrough than expected from modeling. Though clearly not an equilibrium process, retardation involving a dynamic steady state between attachment and detachment was nevertheless a major determinant of transport versus retention of virus in this field experiment.

A series of ground‐water flow and tracer experiments were performed on an undisturbed column of fractured clay‐rich till, 0.5 m diameter by 0.5 m long, in a pressure‐controlled cell. The measured hydraulic conductivity of the sample was 1.0 to 1.2 × 10–6 m/sec and the average hydraulic gradient during the tracer experiments ranged from 0.45 to 0.49. The experiments clearly show that ground‐water flow and contaminant migration through the sample is primarily controlled by fractures and root holes. Tracer experiments using a solute (chloride), colloid‐sized bacteriophage (PRD‐1 and MS‐2) and uncharged latex microspheres, indicated very fast transport rates of 4 to 360 m/day. These rates are similar to fracture flow velocities calculated on the basis of the measured bulk hydraulic conductivity of the column, and measured fracture spacing, using the cubic law for flow through parallel‐walled fractures. Fracture aperture values calculated from the ground‐water flow data (35 to 56 μxm) are of the same magnitude as values calculated from the breakthrough of tracers (13 to 120 μm). Aperture values calculated for fractures (1 to 94 μm) and root holes (2 to 188 μm), on the basis of measured immiscible creosote entry pressures, are also comparable with these values. The injected creosote, a DNAPL, penetrated most of the visible and a few invisible fractures and root holes, indicating that, for this till, fractures and root holes are important conduits for the transport of DNAPL's.

Large quantities of antibiotics are used in agricultural production, resulting in their release to agroecosystems through numerous pathways, including land application of contaminated manure, runoff from manure-fertilized fields, and wastewater irrigation of croplands. Antibiotics and their transformation products (TPs) exhibit a wide range of physico-chemical and biological properties and thus present substantive analytical challenges. Advances in the measurement of these compounds in various environmental compartments (plants, manure, soil, sediment, and water) have uncovered a previously unrealized landscape of antibiotic residues. These advanced multiresidue methods, designed to measure subng g -1 concentrations in complex mixtures, remain limited by the inherent intricacy of the sample matrices and the difficultly in eliminating interferences that affect antibiotic detection. While efficient extraction methods combined with high sensitivity analysis by liquid chromatography/mass spectrometry can provide accurate quantification of antibiotics and their TPs, measured concentrations do not necessarily reflect their bioavailable fractions and effects in the environment. Consequently, there is a need to complement chemical analysis with biological assays that can provide information on bioavailability, biological activity, and effects of mixtures. Enzyme-linked immunosorbent assays (ELISA), often used as screening tools for antibiotic residues, may be useful for detecting the presence of structurally related antibiotic mixtures but not their effects. Other tools, including bioreporter assays, hold promise in measuring bioavailable antibiotics and could provide insights on their biological activity. Improved assessment of the ecological and human health risks associated with antibiotics in agroecosystems requires continued advances in analytical accuracy and sensitivity through improvements in sample preparation, instrumentation, and screening technologies.

BackgroundMineralized and permineralized bone is the most common form of fossilization in the vertebrate record. Preservation of gross soft tissues is extremely rare, but recent studies have suggested that primary soft tissues and biomolecules are more commonly preserved within preserved bones than had been presumed. Some of these claims have been challenged, with presentation of evidence suggesting that some of the structures are microbial artifacts, not primary soft tissues. The identification of biomolecules in fossil vertebrate extracts from a specimen of Brachylophosaurus canadensis has shown the interpretation of preserved organic remains as microbial biofilm to be highly unlikely. These discussions also propose a variety of potential mechanisms that would permit the preservation of soft-tissues in vertebrate fossils over geologic time.Methodology/Principal FindingsThis study experimentally examines the role of microbial biofilms in soft-tissue preservation in vertebrate fossils by quantitatively establishing the growth and morphology of biofilms on extant archosaur bone. These results are microscopically and morphologically compared with soft-tissue extracts from vertebrate fossils from the Hell Creek Formation of southeastern Montana (Latest Maastrichtian) in order to investigate the potential role of microbial biofilms on the preservation of fossil bone and bound organic matter in a variety of taphonomic settings. Based on these analyses, we highlight a mechanism whereby this bound organic matter may be preserved.Conclusions/SignificanceResults of the study indicate that the crystallization of microbial biofilms on decomposing organic matter within vertebrate bone in early taphonomic stages may contribute to the preservation of primary soft tissues deeper in the bone structure.

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A field experiment shows that rapid downward migration of solutes and microorganisms can occur in a fractured till. A solute tracer, chloride, and a bacteriophage tracer, PRD-1, were added to groundwater and allowed to infiltrate downwards over a 4 × 4 m area. Chloride was detected in horizontal filters at 2.0 m depth within 3-40 days of the start of the tracer test, and PRD-1 was detected in the same filters within 0.27 - 27 days. At 2.8 m depth chloride appeared in all the filters, but PRD-1 appeared in only about one-third of the filters. At 4.0 m depth chloride appeared in about one-third of the filters and trace amounts of PRD-1 were detected in only 2 of the 36 filters. Transport rates and peak tracer concentrations decreased with depth, but at each depth there was a high degree of variability. The transport data is generally consistent with expectations based on hydraulic conductivity measurements and on the observed density of fractures and biopores, both of which decrease with depth. Transport of chloride was apparently retarded by diffusion into the fine-grained matrix between fractures, but the rapid transport of PRD-1, with little dispersion, indicates that it was transported mainly through the fractures.

Since 2011, tourism to Mexico’s Yucatán Peninsula has been heavily impacted by large masses of sargassum seaweed washing up on the beaches, with the largest seaweed event occurring in 2019. Seaweed deters beach tourism, potentially shifting tourism inland towards other activities such as swimming in cenotes (sinkholes). Our mixed methods study combined data from surveys of visitors to the region, interviews with tourists and tour operators, thematic analysis of newspaper articles, laws and policies and analysis of water samples from a cenote to understand the environmental impact on cenotes of this shifting tourism industry. We identified intentional efforts by the tourism industry to encourage cenote tourism in response to the seaweed problem, and our survey and interview data confirmed that tourists are choosing to visit cenotes in lieu of beaches. Water samples from one tourist cenote in 2019 indicated increased pollution relative to previous years. Current regulations and management of tourist cenotes are weak, creating the potential for significant long term harm to the environment and to the water sovereignty of surrounding communities. Regulation of cenotes should be strengthened to protect these fragile karst ecosystems and to give local and indigenous residents a formal voice in the management process.

Microbial community composition in selected karst groundwater sites in the Yucatan Peninsula, Mexico, was assessed to determine the environmental variables influencing groundwater microbial diversity. The karst aquifer system is a groundwater-dependent ecosystem and is the world’s second largest underwater karst cave system. The area’s geology allows precipitation to infiltrate into the groundwater system and prevents accumulation of surface water; as such, groundwater is the only source of fresh water on the peninsula. The sampling locations consisted of three karst sinkholes that extend through the freshwater zone into the saline water, and an abandoned drinking water well of an ocean-side resort, during the dry and rainy seasons. The analysis showed that highly diverse microbial communities are present in the Yucatan groundwater, sustained by permanently warm temperatures and high nutrient input from human activity. Proximity to densely populated areas, such as tourist resorts, is the most important factor influencing both the diversity and presence of fecal bacteria and the antibiotic resistance profile.