Breast cancer is a type of cancer with the highest prevalence worldwide. Almost 10–30% of breast cancer cases are diagnosed as positive for HER2 (human epidermal growth factor receptor 2). The currently available treatment methods still exhibit many shortcomings such as a high incidence of side effects and treatment failure due to resistance. This in silico study aims to simulate α-mangostin and chitosan combination conjugated to trastuzumab formulation against HER2 as an effort to improve breast cancer patient therapy. This molecular docking simulation was done through using PatchDock Server. The materials used including the two-dimensional structure of α-mangostin, chitosan, and sodium tripolyphosphate from the PubChem database; trastuzumab FASTA sequence from the DrugBank database; and HER2 structure obtained from a crystal complex with PDB ID: 1N8Z. The results indicated that the particle of α-mangostin and chitosan combinations interacted mostly with the crystallizable fragment (Fc region) of trastuzumab in the conjugation process. The conjugation of trastuzumab to the particle of a combination of α-mangostin and chitosan resulted in the greatest increase in the binding score of the smallest-sized particles (50 Å) with an increase in the score of 3828 and also gave the most similar mode of interaction with trastuzumab. However, the conjugation of trastuzumab eliminated the similarity of the mode of interaction and increased the value of atomic contact energy. Thus, a cominbation of α-mangostin and chitosan conjugated to a trastuzumab formulation was predicted can increase the effectiveness of breast cancer therapy at a relatively small particle size but with the consequence of decreasing atomic contact energy.
Penggunaan psikotropika dan obat mengandung prekursor farmasi (OMPF) tanpa petunjuk atau resep dokter merupakan tindakan di luar indikasi medis yang dapat menyebabkan pengguna mengalami ketergantungan dan menimbulkan gangguan dalam aktivitas sehari-hari akibat pemakaian yang bersifat patologik (menimbulkan kelainan). Oleh karena itu, diperlukan pengawasan terhadap penangan dan penyaluran produk khusus psikotropika dan OMPF salah satunya oleh Pedagang Besar Farmasi (PBF). PBF merupakan suatu perusahaan berbadan hukum yang memperoleh izin dalam proses pengadaan, pelaksanaan penyimpanan, hingga proses penyaluran obat dan/atau bahan obat dengan jumlah yang besar sesuai ketentuan peraturan perundang-undangan. Cara Distribusi Obat yang Baik (CDOB) merupakan pedoman dalam melaksanakan proses penyaluran dan pendistribusian obat atau bahan obat sehingga mutu poduk dapat terjamin sepanjang jalur distribusi. Tujuan: untuk mengetahui kepatuhan pelaksanaan CDOB berdasarkan regulasi pemerintah dan regulasi internal terkait penanganan dan penyaluran produk khusus psikotropika dan OMPF di PBF PT “X” di Kota Tasikmalaya. Metode: Penelitian ini dilakukan secara observasional deskriptif yaitu menggunakan metode observasi langsung dan wawancara dengan menggunakan daftar ceklis kuisioner. Hasil: Perolehan kepatuhan penerapan CDOB terkait produk khusus Psikotropika dan OMPF dari kuisioner yang berjumlah 19 pertanyaan, PBF PT “X” telah memenuhi semua aspek CDOB. Kesimpulan: Berdasarkan hasil observasi, dan wawancara langsung didapatkan kesimpulan penerapan Cara Distribusi Obat yang Baik di PBF PT “X” di Kota Tasikmalaya telah terlaksana dengan sangat baik.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.