Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015–2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses.
Aims: This study aims to identify nucleotide variations in human T-lymphotropic virus type 1 (HTLV-1) proviral genome that might be related with the different clinical conditions associated to the virus. Materials & methods: 91 complete HTLV-1 genomes available in GenBank with their respective clinical information were subjected to in silico analyzes (subtyping, molecular characterization and machine learning). Results: We identified 22 mutations that seems to be important in patients’ clinical condition. The presence of some mutations demonstrated alterations in the proteins physicochemical profile, such as the P34L, present in the p12 protein. Furthermore, a correlation between mutations in long terminal repeat and pX region seems to be important for clinical manifestation. Conclusions: Some mutations have the potential to alter the conformation of viral proteins that are important for infection outcomes. Therefore, further functional studies should be performed to assess the impact of these variations on the pathogenesis and on the development of clinical manifestations associated with HTLV-1.
Human T-lymphotropic virus type 1 (HTLV-1) was the first human retrovirus described. The viral factors involved in the different clinical manifestations of infected individuals are still unknown, and in this sense, sequencing technologies can support viral genome studies, contributing to a better understanding of infection outcome. Currently, several sequencing technologies are available with different approaches. To understand the methodological advances in the HTLV-1 field, it is necessary to organize a synthesis by a rigorous review. This systematic literature review describes different technologies used to generate HTLV-1 sequences. The review follows the PRISMA guidelines, and the search for articles was performed in PubMed, Lilacs, Embase, and SciELO databases. From the 574 articles found in search, 62 were selected. The articles showed that, even with the emergence of new sequencing technologies, the traditional Sanger method continues to be the most commonly used methodology for generating HTLV-1 genome sequences. There are many questions that remain unanswered in the field of HTLV-1 research, and this reflects on the small number of studies using next-generation sequencing technologies, which could help address these gaps. The data compiled and analyzed here can help research on HTLV-1, assisting in the choice of sequencing technologies.
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