NRAMP-1 and iNOS genes were reported to be associated with a defense mechanism against bacteria and virus infections. This study aimed to identify NRAMP-1 and iNOS genes polymorphisms and their associations with the defense mechanisms against Salmonella enteritidis and Newcastle Disease (ND) in SenSi-1 Agrinak chicken. The present study used a total number of 172 SenSi-1 Agrinak chicken. Identifications of NRAMP-1 and iNOS genes polymorphisms were performed by PCR-RFLP method. NRAMP-1 and iNOS genotypes were associated with immunoglobulin Y (IgY) concentration, specific antibodies against S. enteritidis and ND using General Linear Model (GLM). Immunity characteristics were further grouped into high, medium, and low categories. NRAMP-1|SacI exon 11 and iNOS|AluI intron 24 in SenSi-1 Agrinak chickens were polymorphic. TC genotype has a higher immune response to infectious agents compared to TT and CC genotypes. The frequency of C allele was higher than the T allele in the concentration of immunoglobulin Y (IgY), antibodies titers against S enteritidis and ND. The TC genotype of NRAMP-1 gene was significantly associated with ND antibody titers, and the TT genotype of iNOS was significantly associated with S. enteritidis specific antibody. NRAMP-1 and iNOS genes can be used as potential candidate genes for immune traits in SenSi-1 Agrinak chickens.
NRAMP-1 protein and INOS enzyme have been shown as deffense mechanism against bacteria and virus infection. These genes have not been investigated in Sentul Selection chickens. This study was aimed to determine the polymorphism of NRAMP-1 and INOS in Sentul Selected chickens. A number of 172 sentul selected chickens was observed for NRAMP-1 and INOS genes using PCR-RFLP. The frequency of genotypes and alleles, polymorphic information content and Hardy-Weinberg equilibrium status (HWE) were analyzed. Polymorphism in NRAMP1|SacI and INOS|AluI were observed. The population was in HWE for both genes. NRAMP1 gene frequencies had higher TC genotypes and INOS gene frequencies had higher CC genotypes (P<0.05) in Selected Sentul chickens. Furthermore, the NRAMP1 and INOS genes, the frequency of allele C was higher than the T allele. It could be concluded that NRAMP-1 and INOS gene has potential to propose has associated with immune traits in Sentul chicken.
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