In this paper, a rapid and accurate method was developed for the determination of paraquat by a resonance light scattering (RLS) quenching technique with a common spectrofluorometer. In the presence of paraquat, the intensity of resonance light scattering (I RLS ) of DNA-HCL system was significantly quenched. A resonance light scattering peak at 308 nm was found. At this wavelength, the decrease in I RLS was in proportion to the concentration of paraquat. The linear range of the calibration curve was approximately 0.05-1.0 lg mL À1 and the detection limit (S/N ¼ 3) was 0.036 lg mL À1 . The recovery rates of paraquat in river water, rice, and urine samples were determined with satisfactory results. The solid-phase extraction was used for the preparation of urine sample. The spectral characteristics of the ctDNA-paraquat-HCl system were also discussed.
INTRODUTIONResonance light scattering (RLS) phenomenon, as an analytical technique has become the focus of many recent studies for its high sensitivity, simplicity, and rapidity. In 1993, Pasternack found that the enhance RLS signals in an aggregation medium could even be detected by using a common spectrofluorometer and established a method which could characterize the spectral characteristics of molecular aggregation (Pasternack et al. 1993). The RLS technique has proven to be a promising method not only for the study of aggregation and assembly of biological and chemical species, but also for the determination of nucleic acids, proteins, inorganic ions, surfactants, and drugs (Chen et al. 2006).
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