Lodging stress seriously affects the yield, quality, and mechanical harvesting of maize, and is a major natural disaster causing maize yield reduction. The aim of this study was to obtain light detection and ranging (LiDAR) data of lodged maize using an unmanned aerial vehicle (UAV) equipped with a RIEGL VUX-1UAV sensor to analyze changes in the vertical structure of maize plants with different degrees of lodging, and thus to use plant height to quantitatively study maize lodging. Based on the UAV-LiDAR data, the height of the maize canopy was retrieved using a canopy height model to determine the height of the lodged maize canopy at different times. The profiles were analyzed to assess changes in maize plant height with different degrees of lodging. The differences in plant height growth of maize with different degrees of lodging were evaluated to determine the plant height recovery ability of maize with different degrees of lodging. Furthermore, the correlation between plant heights measured on the ground and LiDAR-estimated plant heights was used to verify the accuracy of plant height estimation. The results show that UAV-LiDAR data can be used to achieve maize canopy height estimation, with plant height estimation accuracy parameters of R2 = 0.964, RMSE = 0.127, and nRMSE = 7.449%. Thus, it can reflect changes of plant height of lodging maize and the recovery ability of plant height of different lodging types. Plant height can be used to quantitatively evaluate the lodging degree of maize. Studies have shown that the use of UAV-LiDAR data can effectively estimate plant heights and confirm the feasibility of LiDAR data in crop lodging monitoring.
Grain yield is increasingly affected by climate factors such as drought and heat. To develop resilient and high-yielding cultivars, high-throughput phenotyping (HTP) techniques are essential for precise decisions in wheat breeding. The ability of unmanned aerial vehicle (UAV)-based multispectral imaging and ensemble learning methods to increase the accuracy of grain yield prediction in practical breeding work is evaluated in this study. For this, 211 winter wheat genotypes were planted under full and limited irrigation treatments, and multispectral data were collected at heading, flowering, early grain filling (EGF), and mid-grain filling (MGF) stages. Twenty multispectral vegetation indices (VIs) were estimated, and VIs with heritability greater than 0.5 were selected to evaluate the models across the growth stages under both irrigation treatments. A framework for ensemble learning was developed by combining multiple base models such as random forest (RF), support vector machine (SVM), Gaussian process (GP), and ridge regression (RR). The R2 values between VIs and grain yield between for individual base models were ranged from 0.468 to 0.580 and 0.537 to 0.598 for grain yield prediction in full and limited irrigation treatments across growth stages, respectively. The prediction results of ensemble models were ranged from 0.491 to 0.616 and 0.560 to 0.616 under full and limited irrigation treatments respectively, and were higher than that of the corresponding base learners. Moreover, the grain yield prediction results were observed high at mid grain filling stage under both full (R2 = 0.625) and limited (R2 = 0.628) irrigation treatments through ensemble learning based stacking of four base learners. Further improvements in ensemble learning models can accelerate the use of UAV-based multispectral data for accurate predictions of complex traits like grain yield in wheat.
Crop traits such as aboveground biomass (AGB), total leaf area (TLA), leaf chlorophyll content (LCC), and thousand kernel weight (TWK) are important indices in maize breeding. How to extract multiple crop traits at the same time is helpful to improve the efficiency of breeding. Compared with digital and multispectral images, the advantages of high spatial and spectral resolution of hyperspectral images derived from unmanned aerial vehicle (UAV) are expected to accurately estimate the similar traits among breeding materials. This study is aimed at exploring the feasibility of estimating AGB, TLA, SPAD value, and TWK using UAV hyperspectral images and at determining the optimal models for facilitating the process of selecting advanced varieties. The successive projection algorithm (SPA) and competitive adaptive reweighted sampling (CARS) were used to screen sensitive bands for the maize traits. Partial least squares (PLS) and random forest (RF) algorithms were used to estimate the maize traits. The results can be summarized as follows: The sensitive bands for various traits were mainly concentrated in the near-red and red-edge regions. The sensitive bands screened by CARS were more abundant than those screened by SPA. For AGB, TLA, and SPAD value, the optimal combination was the CARS-PLS method. Regarding the TWK, the optimal combination was the CARS-RF method. Compared with the model built by RF, the model built by PLS was more stable. This study provides guiding significance and practical value for main trait estimation of maize inbred lines by UAV hyperspectral images at the plot level.
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