Rifampin (RIF) is a bactericidal antibiotic drug and potent inducer of hepatic and intestinal cytochrome P-450 (CYP-450) enzyme systems. Given by mouth or intravenously, it can cause numerous clinical drug interactions; thus, alternative systems of drug delivery that bypass some or all of its toxic effects are well worth investigating. In this study, a controlled layer-by-layer (LBL) process of encapsulating RIF in biocompatible alginate and chitosan polymers loaded onto Fe O nanoparticles was developed. Fe O nanoparticles were synthesized from FeCl ·6H O using a hydrothermal procedure. Fluorescent molecular beacons containing RIF molecules and Texas Red were loaded onto the surfaces of Fe O nanoparticles. The loaded nanoparticles were encapsulated in alginate and chitosan layers with alternating negative and positive surface charge using an LBL self-assembly method. Subsequently, by removing the Fe O template particles, polymeric capsules containing RIF were obtained. Ultraviolet-visible spectrophotometry employed to determine optimized conditions for loading RIF, measure the amount of RIF loaded onto the surface of the nanoparticles under optimized conditions, and study drug-release capability. Scanning electron microscopy was used to characterize the morphology of unloaded and loaded nanoparticles. X-ray diffraction and Fourier transform infrared spectroscopy were applied to demonstrate the production of nanoparticles and loading of RIF onto them. Zeta potential analysis was used to determine the size and surface potential of the loaded polymeric layers. After removal of the core template, confocal fluorescence microscopy was used to isolate polymeric capsules containing RIF. The average size of the nanoparticles obtained was 23 nm. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 905-913, 2018.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.