Long non-coding RNAs (lncRNAs) are involved in various pathophysiologic processes and human diseases. However, their dynamics and corresponding functions in pulmonary fibrosis remain poorly understood. In this study, portions of lncRNAs adjacent or homologous to protein-coding genes were determined by searching the UCSC genome bioinformatics database. This was found to be potentially useful for exploring lncRNA functions in disease progression. Previous studies showed that competing endogenous RNA (ceRNA) hypothesis is another method to predict lncRNA function. However, little is known about the function of ceRNA in pulmonary fibrosis. In this study, we selected two differentially expressed lncRNAs MRAK088388 and MRAK081523 to explore their regulatory mechanisms. MRAK088388 and MRAK081523 were analysed as long-intergenic non-coding RNAs (lincRNAs), and identified as orthologues of mouse lncRNAs AK088388 and AK081523, respectively. qRT-PCR and in situ hybridization (ISH) showed that they were significantly up-regulated, and located in the cytoplasm of interstitial lung cells. We also showed that MRAK088388 and N4bp2 had the same miRNA response elements (MREs) for miR-200, miR-429, miR-29, and miR-30, whereas MRAK081523 and Plxna4 had the same MREs for miR-218, miR-141, miR-98, and let-7. Moreover, the expression levels of N4bp2 and Plxna4 significantly increased in fibrotic rats, and were highly correlated with those of MRAK088388 and MRAK081523, respectively. Among their shared miRNAs, miR-29b-3p and let-7i-5p decreased in the model group, and were negatively correlated with the expression of MRAK088388 and MRAK081523, respectively. MRAK088388 and MRAK081523 could regulate N4bp2 and Plxna4 expression by sponging miR-29b-3p and let-7i-5p, respectively, and possessed regulatory functions as ceRNAs. Thus, our study may provide insights into the functional interactions of lncRNA, miRNA and mRNA, and lead to new theories for the pathogenesis and treatment of pulmonary fibrosis.

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Using a dataset extended by the addition of data for 2004-08, this study reexamined the trend in the sensible heating (SH) flux at 73 meteorological stations over the Tibetan Plateau (TP) during 1980-2008 and investigated its impact on monsoon precipitation in the surrounding region. In contrast to ongoing climate warming, a weakening trend in SH is persistent over most of the plateau, despite a sharp increase in the ground-air temperature difference in 2004-08. The weakening trend in SH over the TP is primarily a response to the spatial nonuniformity of large-scale warming over the East Asian continent, which is characterized by much greater warming amplitude at mid-and high latitudes than over the tropics and subtropics. Furthermore, the suppressed air pump effect, which is driven by SH over the TP and acts as a strong forcing source, gives rise to reduced precipitation along the southern and eastern slopes of the plateau, and increased rainfall over northeastern India and the Bay of Bengal. No significantly stable correlation exists between the SH source over the TP and the overall trend or interdecadal variability in the East Asian or South Asian summer monsoon.