Improving biosensing performance is mandatory for biomolecular recognition and disease identification. Gold nanoparticle (GNP)‐based colorimetric assay is the easy and cost‐effective identification method by a naked eye detection. In this research, osteosarcoma biomarker (miRNA‐195) was identified by citrate‐capped GNP‐colorimetric assay. As salt‐induced aggregation was used to observe the color changes of GNP, sodium chloride (NaCl) and capture DNA were optimized as 50 mM and ∼20 pmol, respectively. The capture DNA only on GNP could not stabilize under high NaCl, and the color of GNP turned into purple. At the same time, when capture DNA was hybridized with target, the condition can stabilize the GNP under higher NaCl, which retains the GNP color as red. This simple assay reaches the limit of detection of target miRNA‐195 as ∼40 fmol. Control experiments with noncomplementary DNA turned the solution into purple, indicating the specific detection of target. The mixture of target in diluted serum retains the color of the GNP solution to be red, indicating the selective detection of target DNA. This simple assay helps to quantify the level of miRNA‐195 target DNA and to diagnose the osteosarcoma.
Wang J, Xie D, Cai Z, et al. Does a home-based exercise program play any role in the treatment of knee osteoarthritis? A meta-analysis [published online as ahead of print on August 24, 2022].
This work aimed to analyze the diagnostic value of dynamic scanning of multislice spiral computed tomography (MSCT) and magnetic resonance imaging (MRI) for benign and malignant bone tumor and nursing intervention. 108 patients with bone tumor were selected as the research objects, all of which underwent MSCT and MRI scans. The accuracy, sensitivity, and specificity of MSCT, MRI, and MSCT + MRI for identifying benign and malignant bone tumors and nursing care were calculated, as well as the diagnostic accuracy of MSCT, MRI, and MSCT + MRI for different bone tumor pathological types. The results showed that the accuracy of MSCT + MRI (97.56%) in distinguishing benign and malignant bone lesions was remarkably higher relative to that of MSCT (85.91%) and MRI (89.85%) (
P
<
0.05
). The sensitivity and specificity of MSCT + MRI (94.85%; 90.52%) in distinguishing benign and malignant bone lesions were obviously greater in contrast to those of MSCT (83.66%; 79.05%) and MRI (86.02%; 81.17%) (
P
<
0.05
). The malignant misdiagnosis rate and malignant missing report rate of MSCT + MRI in distinguishing benign and malignant bone lesions were inferior to those of MSCT and MRI notably (
P
<
0.05
). The accuracy of MSCT + MRI in distinguishing osteosarcoma, giant-cell tumor of bone (GCT), bone cyst, and osteofibrous dysplasia (OFD) was evidently higher versus that of MSCT and MRI (
P
<
0.05
). The accuracy of MSCT + MRI in distinguishing osteofibroma and ganglioneuroma was greatly higher than that of MSCT and MRI (
P
<
0.05
). The accuracy of MSCT + MRI in distinguishing osteofibroma and ganglioneuroma was 68.64% and 71.63%, respectively. In short, in contrast to the single MSCT and MRI examination, MSCT combined with MRI detection can effectively improve the accuracy of judgment for benign and malignant bone tumor lesions and nursing care and had higher sensitivity and specificity. MSCT combined with MRI had better performance in identifying osteosarcoma, GCT, bone cyst, and OFD but poor performance in osteofibroma and ganglioneuroma.
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