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In this study, simulation software AnyLogic was used to establish a station simulation model for a metro line. First, a basic model of the environment of the metro station was drawn, and accordingly, reasonable assumptions and simplifications were proposed. Then, a diagram of the passenger walking path was created and the simulation variables and functions for passenger flow management were designed. Considering Youfangqiao Station of Nanjing Metro Line 2 in China as an example, the real passenger flow data of this station were statistically analyzed. To simulate the station passenger flow management, input parameters such as the passenger space diameter, passenger flow generation rate, delay rate of automatic fare collection equipment and security check machine, and the number of gates were considered. Passenger flow management was optimized for the morning and evening peak periods, and reasonable suggestions were proposed based on the optimization results, providing a theoretical basis for the construction planning and preevaluation of station operation capacities of urban rail transit systems.

Elucidating the effect of γ-aminobutyric acid (GABA) on seed germination and seedling growth of Medicago ruthenica under low-temperature could provide a reference for alleviating the harmful effects of low temperatures on legumes in alpine meadows. We set up different temperatures to screen three low temperatures that inhibit seed germination of Medicago ruthenica, and six aqueous concentrations of GABA (0, 0.1, 0.5, 1, 5, 10 mM) were set up to screen out the best GABA seed soaking concentration that can promote the seed germination at low-temperature.The three temperatures of 10 C, 20/5 C and 15/5 C inhibited seed germination of Medicago ruthenica. Soaking seeds with 1 mM GABA could significantly increase seed germination rate of Medicago ruthenica at low-temperature (10 C). Soaking seeds only with 0.1 mM GABA could promote germination of Medicago ruthenica at 20/5 C, but there is no significant difference compared with distilled water soaking.Whereas, all GABA seed soaking concentrations delayed seed germination time and inhibited seed germination at 15/5 C. Pre-spraying seedlings with 1 mM GABA at 10 C reduced the production of superoxide anion, hydrogen peroxide and malondialdehyde content, and increased the activities of catalase, peroxidase, superoxide dismutase and ascorbate peroxidase in leaves of Medicago ruthenica on days 7. Simultaneously, Pre-spraying seedlings with 1 mM GABA at 10 C significantly increased the net photosynthetic rate and decreased intercellular CO 2 concentration. These data suggest that GABA could enhance the cold tolerance of Medicago ruthenica by promoting seed germination, regulating the antioxidant system, and increasing photosynthetic efficiency. However, the mitigation effect of GABA on low-temperature is only applicable to a certain temperature. The mitigation effect of GABA on low temperature will be weakened as the low temperature of plants is less than 10 C