This work elucidated the valence states effect on the electrogenerated chemiluminescence (ECL) performance of gold nanocluster (AuNC). The N-acetyl-l-cysteine-AuNCs (NAC-AuNCs) and the electrochemical reduction method for reducing the AuNCs were first employed to this study. Results demonstrate that the electrochemical reduction degree of the AuNCs depended on the reduction potential, and the enhancement of the ECL signals was positively correlated with the reduction degree of AuNCs, which indicated that the valence state of Au plays a vital role in the ECL performance of AuNCs. Furthermore, the proposed method has been successfully extended to the chemical reduction technique and other nanoclusters. Therefore, an excellent AuNC-based ECL method with various advantages, such as simple preparation, lower toxicity, high sensitivity, and Φ, and excellent stability, has been proposed. This approach not only opens up a new avenue for designing and developing ECL device from other functional-metal based NCs, but also extends the huge potential application in the ECL sensing.
Seeking
for an advanced electrochemiluminescence (ECL) platform
is still an active and continuous theme in the ECL-sensing realm.
This work outlines a femtomolar-level and highly selective glutathione
(GSH) and adenosine triphosphate (ATP) ECL assay strategy using a
facile split-type gold nanocluster (AuNC) probe-based ECL platform.
The system utilizes GSH as an efficient etching agent to turn on the
MnO2/AuNC-based ECL nanoswitch platform. This method successfully
achieves an ultrasensitive detection of GSH, which significantly outperformed
other sensors. Based on the above excellent results, GSH-related biological
assays have been further established by taking ATP as a model. Combined
with the high catalytic oxidation ability of DNAzyme, this ECL sensor
can realize ATP assay as low as 1.4 fmol without other complicated
exonuclease amplification strategies. Thus, we successfully achieved
an ultrahigh sensitivity, extremely wide dynamic range, great simplicity,
and strong anti-interference detection of ATP. In addition, the actual
sample detection for GSH and ATP exhibits satisfactory results. We
believe that our proposed high-performance platform will provide more
possibilities for the detection of other GSH-related substances and
show great prospect in disease diagnosis and biochemical research.
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