The Genotype-Tissue Expression (GTEx) project was established to characterize genetic effects on the transcriptome across human tissues and to link these regulatory mechanisms to trait and disease associations. Here, we present analyses of the version 8 data, examining 15,201 RNA-sequencing samples from 49 tissues of 838 postmortem donors. We comprehensively characterize genetic associations for gene expression and splicing in cis and trans, showing that regulatory associations are found for almost all genes, and describe the underlying molecular mechanisms and their contribution to allelic heterogeneity and pleiotropy of complex traits. Leveraging the large diversity of tissues, we provide insights into the tissue specificity of genetic effects and show that cell type composition is a key factor in understanding gene regulatory mechanisms in human tissues.

A prominent interspecific pattern of sexual size dimorphism (SSD) is Rensch's rule, according to which male body size is more variable or evolutionarily divergent than female body size. Assuming equal growth rates of males and females, SSD would be entirely mediated, and Rensch's rule proximately caused, by sexual differences in development times, or sexual bimaturism (SBM), with the larger sex developing for a proportionately longer time. Only a subset of the seven arthropod groups investigated in this study exhibits Rensch's rule. Furthermore, we found only a weak positive relationship between SSD and SBM overall, suggesting that growth rate differences between the sexes are more important than development time differences in proximately mediating SSD in a wide but by no means comprehensive range of arthropod taxa. Except when protandry is of selective advantage (as in many butterflies, Hymenoptera, and spiders), male development time was equal to (in water striders and beetles) or even longer than (in drosophilid and sepsid flies) that of females. Because all taxa show female-biased SSD, this implies faster growth of females in general, a pattern markedly different from that of primates and birds (analyzed here for comparison). We discuss three potential explanations for this pattern based on life-history trade-offs and sexual selection. Online enhancement: appendix table.abstract: A prominent interspecific pattern of sexual size dimorphism (SSD) is Rensch's rule, according to which male body size is more variable or evolutionarily divergent than female body size. As-

One of the main concerns about the commercial release of transgenic crops is the likelihood of transgene spread from cultivated species into wild relatives. This question is relevant for oilseed rape/canola (Brassica napus, AACC, 2n=38), as this species is partially allogamous with several wild relatives that are often sympatric with oilseed rape production. A workshop sponsored by the European Science Foundation (11-13 June 2001, Rennes, France) was held: (i) to identify the main weeds present in European and North American countries; (ii) to review results on the ability of oilseed rape to hybridize and backcross with wild relatives; (iii) to review the usefulness and limitations of the tools available for monitoring interspecific hybridization and gene introgression; and (iv) to provide recent results on modelling of gene flow.

The effect of various combinations of vitamins C and E on immune response and disease resistance was investigated in rainbow trout, Oncorhynchus mykiss (Walbaum). The following combinations of vitamins were included in the diet (C/E): 0/0, 0/800, 2000/0, 30/30, 30/800, 2000/30, 2000/800. Liver ascorbic acid and alpha-tocopherol contents reflected the dietary treatments well. Complement levels showed no significant differences. The combination of the high dietary doses of vitamins C and E significantly stimulated lymphoproliferation induced by Concanavalin A when compared with fish fed a low level of both vitamins, but also when compared with fish fed a low level of vitamin C and a high level of vitamin E. The highest macrophage oxidative burst activity, measured by chemiluminescence, was found in fish receiving both vitamins at high levels. In the disease resistance experiments, the best survival rates in trout infected with viral haemorrhagic septicaemia virus were achieved with diets containing both vitamins at a high level, or at least one at a high and the other at a low level. Highest mortalities in fish infected with Yersinia ruckeri occurred in trout fed double-deficient or double low diets. The best survival of fish exposed to Ichthyophthirius multifiliis was achieved with diets high in one and low in the other, or high in both vitamins. However, differences were not statistically significant in the parasite experiment.

We investigate a novel waveguide geometry consisting of a high dielectric medium adjacent to a metal plane with a thin low dielectric spacer. The mechanism of operation is explained and simulation results are presented.Surface plasmons (SPs), i.e. charge density oscillations along metallic interfaces, have the ability to confine and guide electromagnetic energy below the so called diffraction limit and hence offer the potential of dense integration for photonic devices. In recent years, a variety of SP waveguides have been proposed [1][2][3]. In this paper we investigate the properties of a new kind of waveguide consisting of a lossy dielectric nanowire adjacent to a metallic surface. Electromagnetic radiation is guided in a super mode resulting from the coupling between a SP mode and a dielectric waveguide-mode. We analyze the confinement of the guided mode and the propagation losses. It is shown that the proposed structure shows very good confinement and propagation distances even considering the high loss of the dielectric medium.

We introduce the TinyNode platform for wireless sensor networks. Supporting both research and industrial deployments, the platform offers communication ranges that exceed current platforms by a factor of 3 to 5, while consuming similar energy. It comes with a rich, practical set of hardware extensions and full TinyOS support. We describe the design choices of the TinyNode, the accompanying hardware modules, and the MAC layer implementation.

Abstract-This review is an attempt to untangle the complexity of transtibial prosthetic socket fit and perhaps find some indication of whether a particular prosthetic socket type might be best for a given situation. In addition, we identified knowledge gaps, thus providing direction for possible future research. We followed the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines, using medical subject headings and standard key words to search for articles in relevant databases. No restrictions were made on study design and type of outcome measure used. From the obtained search results (n = 1,863), 35 articles were included. The relevant data were entered into a predefined data form that included the Downs and Black risk of bias assessment checklist. This article presents the results from the systematic review of the quantitative outcomes (n = 27 articles). Trends indicate that vacuum-assisted suction sockets improve gait symmetry, volume control, and residual limb health more than other socket designs. Hydrostatic sockets seem to create less inconsistent socket fittings, reducing a problem that greatly influences outcome measures. Knowledge gaps exist in the understanding of clinically meaningful changes in socket fit and its effect on biomechanical outcomes. Further, safe and comfortable pressure thresholds under various conditions should be determined through a systematic approach.

The Magallanes–Tierra del Fuego region, Southern Patagonia (53–56°S) features a plethora of fjords and remote and isolated islands, and hosts several thousand glaciers. The number of investigated glaciers with respect to the multiple Neoglacial advances is based on a few individual studies and is still fragmentary, which complicates the interpretation of the glacial dynamics in the southernmost part of America. Schiaparelli Glacier (54°24′S, 70°50′W), located at the western side of the Cordillera Darwin, was selected for tree-ring-based and radiocarbon dating of the glacial deposits. One focus of the study was to address to the potential dating uncertainties that arise by the use of Nothofagus spp. as a pioneer species. A robust analysis of the age–height relationship, missing the pith of the tree (pith offset), and site-specific ecesis time revealed a total uncertainty value of ±59 years. Three adjacent terminal moraines were identified, which increasingly tapered towards the glacier, with oldest deposition dates of 1749 ± 5 CE, 1789 ± 5 CE, and 1867 ± 5 CE. Radiocarbon dates of trunks incorporated within the terminal moraine system indicate at least three phases of cumulative glacial activity within the last 2300 years that coincide with the Neoglacial phases of the Southern Patagonian Icefield and adjacent mountain glaciers. The sub-recent trunks revealed the first evidence of a Neoglacial advance between ~600 BCE and 100 CE, which so far has not been substantiated in the Magallanes–Tierra del Fuego region.