L (liver) type phosphofructokinase subunits purified from human leukocytes are
slightly lighter than L subunits from liver and red blood cells. A mild treatment of red blood
cell L4 enzyme with subtilisin converts its subunits into forms of similar molecular weight to
leukocyte enzyme. From a kinetical point of view, subtilisin-treated L(4) phosphofructokinase
and leukocyte enzymes are characterized by a decrease of the allosteric properties as compared
to non-treated red cell L(4) phosphofructokinase.
A new deficient variant of glucose-6-phosphate
dehydrogenase, G6PD Baudelocque, giving rise to
severe congenital non-spherocytic hemolytic disease is
described. It is characterized by fast electrophoretic mobility,
extreme in vitro and in vivo lability, normal K(m)
for G6P, normal utilization of analogues, and normal pH
curve. Because of transfusions, most of the investigations
were carried out in cultured fibroblasts derived from a
skin biopsy.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.