Lycopene is present in a range of fresh fruits and vegetables, especially in the leaves of Barringtonia racemosa. The traditional lycopene extraction from the plant is being employed instead of an easy propagation technique like cell culture process from the leaf explants. We intend to assess how lycopene could be extracted via tissue culture under light (illuminance: 8,200 lux under white fluorescent lamps, photoperiod 16 h per day at 25°C) and dark. Leaf explants of Barringtonia racemosa were cultured on modified Murashige and Skoog (MS), Woody Plant Medium (WPM) and B5 media, supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). Optimal conditions for callus induction and maintenance under both dark and light were investigated, and growth and lycopene accumulation were evaluated. Among media with different concentrations of 2,4-D, fast growing, friable callus initiated within three weeks after culturing on WPM basal medium supplemented with 2.0 mg L -1 (weight per volume) of 2,4-D, whereas callus induction in explants cultured on all other media started only after five weeks. Calli were subcultured once every fortnight. Pale yellow and green calli developed under conditions of dark and light respectively were then selected for evaluation of their lycopene contents. An improved reversed phase of high performance liquid chromatography (HPLC) method was used for a selective chemical determination of the lycopene content. Light induced lycopene production; and likewise maximum lycopene level incubated in light was higher than those incubated in darkness. The best growth rates of callus and cell suspension were achieved in WPM and B5 media respectively. The production of lycopene was growth-dependent through analysis of growth and lycopene content of both callus and cell suspension cultures. Key words: Powderpuff tree, carotene, phytochemical, plant micropropagation, growth hormones Otimização de culturas de suspensões de calos e células de Barringtonia racemosa (família Lecythidaceae) para produção de licopeno RESUMO: O licopeno está presente numa série de frutas frescas e hortaliças principalmente na folhas de Barringtonia racemosa. A extração tradicional do licopeno tem sido empregada no lugar da fácil técnica de propagação como o processo de cultura de células de explantes de folhas. É nossa intenção demonstrar como o licopeno pode ser extraído através de cultura de tecido sob luz (iluminação com lâmpadas fluorescentes brancas de 8.200 lux, 16 h por dia a 25° C) e escuro. Explantes de folhas de Barringtonia racemosa foram cultivados em meio modificado de Murashige e Skoog (MS) para plantas lenhosas e meio B5, suplementado com diferentes concentrações de ácido 2,4-Diclorofenoxiacético (2,4-D). Condições ótimas para indução e manutenção de calos sob luz e escuro foram investigadas e avaliados o crescimento e acumulo de licopeno. Entre meios com diferentes concentrações de 2,4 -D, calos friáveis de crescimento rápido tiveram início em três semanas após serem cultivados em...

Background:The previous studies showed that herpes human virus-6 (HHV-6) and HHV-7 exist in salivary glands. One of the important areas in oral and maxillofacial pathology field is tumors of the salivary glands. In this study, to declare the major sites of persistent infection with HHV-6 and HHV-7, the existence of HHV-6 and HHV-7 genomes in formalin-fixed paraffin embedded tissue samples of salivary gland tumors.Methods:This analytical study was performed in 60 paraffin blocks samples of malignant and benign neoplasms of both major and minor salivary glands. This study performed with highly sensitive real time PCR method.Results:Among 60 paraffin blocks salivary gland tumors with equal chances of presence of the HHV-7 and HHV-6 in the samples, 34% were positive for both HHV-7 and HHV-6 while 47.2% were only positive for HHV-7, 18.9% samples were positive for HHV-6. A relationship was noticed between HHV-7 and HHV-6 genomes.Conclusion:In conclusion, this study showed no relation between virus and diseases with P=0.953. Also it could be inferred that there is a relationship between HHV-6 and 7 in salivary glands neoplasms.

The seeds of S. securidaca were collected from Agriculture and Natural Recourses Research Center of Isfahan, Iran, in December 2009. The specimen was identified by Prof. Rahimnejad and deposited at the University of Isfahan herbarium, Iran (No. 13656). Plant seeds (2 kg) were carefully dried in a well-ventilated dark room and powdered. Finally, the dried seed powder (1 kg) was obtained. Extraction and isolation of compounds Methanol (98%) extracts of dried and powdered S. securidaca seeds (1000 g) were prepared. The extraction was done thrice at 40°C. Then, the resulting liquid was collected, filtered and reduced through evaporation under vacuum by a rotary evaporator (Stroglass, Italy) at 45°C and dried by use of a freeze dryer (Zirbus, Germany). Silica-gel column fractionation chromatography was carried out with the dried

Background:Although cervical uterine cancer is a second form of cancer among women, but it occupies fifth form among all cancer types.Methods:In the present study, human papillomavirus (HPV) and herpes simplex virus 2 (HSV-2) in cervical cancer patients by using real time polymerase chain reaction (PCR) technique and the relation between their viral loads were investigated. 156 cervical carcinoma tissues were collected from married women in health centers in Isfahan, Iran.Results:The results showed that among 156 specimens, 58.97%, 45.51% and 7.05% were positive for HPV DNA, HPV-16 and HPV-18 respectively. Only in 2.3% specimens, HSV-2 and HPV-16 were positively detected where viral load HSV-2 in conjunction with HSV-16 dramatically increased.Conclusion:Thus the present study not only confirmed that viral load of HPV-16 is more than other HPV types, but also in possible conjunction with HSV-2, both rates will significantly increase.