In this research, the effects of different temperatures (8, 10, 12, 14, 16 and 18°C) and salinities (5, 10, 15, 18, 20, 25, 30, 35 and ‰40) on the development of turbot (Psetta maxima) eggs were investigated in triplicates. Broodstock produced in CFRI were used in this research. Fertilization, hatching, anomaly rate and larvae length were measured. Time dependent embryonic development were observed.Embryonic development was completed in each temperatures. It is observed that water temperature has significant effects on fertilization, hatching, anomaly and larvae length (P<0.05). Regression analysis showed that there was a negative correlation between the embryonic development and the water temperature of incubation. Time for hatching at 8 and 18°Cwere 2552 hour/degree and 1350 hour/degree, respectively. Better fertilization rate were obtained in salinities of ‰18, ‰20 and ‰25, respectively. Fertilization was not succeeded in the salinity values of 5‰ and ‰10. The anomaly rate in the salinity values of 15‰ and 40‰ was higher then in accordance with other salinity values.According to result of this study, to get high fertilization and haching rate and low abnormality for Black Sea Turbot, water temperature and water salinity were found as 14°C and ‰18, respectively.
In this study, gonadal changes of dusky grouper Epinephelus guaza L.1758 were investigated by histo-anatomically according to sexual maturity in Antalya Bay of Eastern Mediterranean in Turkey. The specimens (n=104) were captured monthly by spear-fishing from their natural sites. The gonads of them were dissected as soon as and fixed in 10 % neutral buffered formaldehyde and Bouin's solution. They were embedded in paraffin blocks after several histo-chemical processes. Sectioned tissue samples at 5µm were stained by Haematoxylin-eosin and Mallory trichrome techniques. Mainly four different stages which were immature stage, mature female stages (from F1 to F6), transitional stages (from T1 to T3), and mature male stages (from M1 to M3) were observed according to gonadal development. However GSI (Gonadosomatik indeks) is the most common used to quantify spawning time in fish; the RGI (Relative gonadal index) was found more appropriate than GSI for the fish. Dusky groupers spawned in June and September in Antalya Bay.
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