Abstract:One of the main mechanisms of action for photodynamic therapy (PDT) is the destruction of tumor vasculature. We observed the PDT-induced vasculature destruction in a mouse model of skin cancer using two techniques: Photoacoustic microscopy (PAM) and diffuse correlation spectroscopy (DCS). PAM showed high-resolution images of the abnormal microvasculature near the establishing tumor area at pre-PDT, as well as the subsequent destruction of those vessels post-PDT. DCS indicated a significant blood flow decrease after PDT, confirming the vascular destruction. Noninvasive assessment of vascular changes may be indicative of therapy response.

composed of small basaloid cells without atypia in a densely collagenous stroma. Horn cysts and focus of calcification were seen within some of the islands. These features confirmed the diagnosis of DTE ( Figure 4A). DiscussionThis case not only supports the previously reported dermoscopic, RCM, and histological features of a DTE but also provides novel insights into its morphological features using HD-OCT. The low-reflective dermal tumor islands (slice and en face) correlate with the dark tumor islands seen in RCM and also with the strands of basaloid cells observed in histology. 3,4 The surrounding highly reflective collagen and dilated vessels in HD-OCT correspond to the same structures presented in the desmoplastic stroma both in RCM and histopathology. The presence of these features, the connection between some islands and follicles, and also the absence of epidermal disarray, inflammatory cells, and peripheral dark rim, which are usually seen in BCC, allowed to favor the diagnosis of DTE over BCC in HD-OCT. 5 In conclusion, this case demonstrated an excellent correlation between HD-OCT, RCM, and histopathology. To the best of the authors' knowledge, this is the first report of HD-OCT imaging of rare DTE. Recognition of such features may facilitate its in vivo diagnosis, avoiding invasive diagnostic techniques of an otherwise benign tumor frequently located in cosmetically sensitive areas.

Today, phototherapy devices are used to treat newborn jaundice. However, there are some cases that the commercial phototherapy devices are inadequate. Most of the device are controlled only at the certain interval that prevents instantaneous monitoring the newborn's vital parameters. For other cases, devices are one-way lighting. In addition, when the baby is taken away from the device, unnecessary lighting causes the decrease in LED's lifetime. In the scope of the study, intensive phototherapy device was developed to eliminate deficiencies that are mentioned above. Properties of the developed phototherapy device are; Automatic detection of newborn's presence, double-sided illumination, continuous monitoring with IP webcam, temperature measurement of the environment and also newborn's body temperature. The light intensity of the device was measured with a photometer as 28.6 μW/cm²/nm that was obtained from 460nm wavelength LED illumination in both top and bottom in accordance with the American Academy of Pediatrics (AAP) guideline.

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