We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots and diluted at 37 °C with base extenders containing lycopene (1 × 10(-3) g ml(-1) ) and resveratrol (1 mm), and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The supplementation of the semen extender with lycopene and resveratrol increased the percentages of post-thawed computer-assisted sperm analysis (CASA) motility (55.8 ± 3.8 and 61.9 ± 4.0%) and progressive motility (38 ± 2.4 and 37 ± 8.8), compared with the controls (50.7 ± 2.65 and 33.3 ± 3.74%, respectively, P < 0.05). Resveratrol provided a higher ALH (4.3 ± 0.1), in comparison with the control (3.9 ± 0.3, P < 0.05). The supplementation of the semen extender with lycopene and resveratrol produced a higher mitochondrial activity (24.6 ± 2.9 and 30.1 ± 6.5% respectively), compared with that of the control (11.8 ± 9.5%, P < 0.05). It was determined that both antioxidants resulted in a lower percentage of sperm with damaged DNA than that of the control (P < 0.05). Sperm motion characteristics except for ALH, acrosome integrity, sperm viability and oxidative stress parameters were not affected by the adding of lycopene and resveratrol.
Ultrasonographic appearance of the testis and epididymis, and seminal characteristics, with regard to localization of spermatic granuloma were studied. In rams with spermatic granuloma (n = 9), diagnosed by clinical or ultrasonographic examinations before histopathological confirmation, localization of each lesion was recorded. Epididymal granulomas, caput and cauda, were seen as anechoic or hyperechoic areas with a distinct margin with or without a hyperechoic capsule. Granulomas in the testis were microscopic and, therefore, could not be detected by ultrasonography. Enlargement in the mediastinum testis was detected in all rams when there were granulomas in the caput. Heterogeneous testis parenchyma invaded by numerous hyperechoic foci, representing testicular degeneration, was associated with granulomas both in the epididymis and testis. Ultrasonographic appearance of the lesions did not differ with regard to season. Seminal characteristics varied between rams. It was concluded that ultrasonographic evaluation may give valuable information in the diagnosis of sperm granuloma in the ram.
Makale Kodu (Article Code): KVFD-2009-429
ÖzetBu çalışmada, geçiş dönemi başındaki koyunlarda farklı progesteron ve PGF2α uygulamalarının, östrüs senkronizasyonu ve ovaryum aktivitesini uyarmadaki etkisi araştırıldı. Araştırmada toplam 75 koyun kullanıldı ve koyunlar 5 gruba ayrıldı. Birinci gruptaki koyunlara (n=15) 30 mg flourogesterone acetate (FGA-30), ikinci gruptaki koyunlara (n=15) 40 mg flourogestene acetate (FGA-40) içeren süngerler vagina içine yerleştirildi. Her iki gruptaki süngerler 12 gün sonra uzaklaştırıldı. Üçüncü gruptaki koyunlara (n=15) 3 mg Norgostomet içeren kulak implantı (N-İMPLANT) kulak derisi altına yerleştirildi ve 9 gün sonra uzaklaştırıldı. Dördüncü gruptaki koyunlara (n=15) Tiaprost tromethamine (PGF2α; 0.294 mg) 9 gün arayla iki kez kas içi yolla enjekte edildi. Beşinci grup (n=15) kontrol olarak oluşturuldu. Vaginal süngerlerin, implantların çıkarılmasını ve ikinci PGF2α enjeksiyonunu takiben tüm çalışma gruplarındaki koyunlara kas içi yolla 600 IU PMSG enjekte edildi. Östrüste oldukları belirlenen koyunlara doğal aşım yaptırıldı. Östrüs görülme oranları FGA-30 ve N-İMPLANT gruplarında (%93.3), FGA-40 grubunda (%86.6) ile PGF2α (%53.3) ve Kontrol (%26.6) gruplarına göre istatistiki açıdan önemli şekilde yüksek belirlendi. Gebelik oranları ise FGA-30 (%93.3) ve N-İMPLANT (%93.3) gruplarında, PGF2α (%53.3) grubuna göre belirgin olarak yüksek bulundu. Kuzulama oranı açısından deneme grupları arasında önemli bir farklılık gözlenmedi. Çoklu doğum oranı N-İMPLANT grubunda (%35.7), PGF2α (%0.0) grubuna göre belirgin olarak yüksek bulundu. Sonuç olarak, geçiş dönemi başındaki koyunlarda PGF2α'nın çift doz uygulanmasıyla farklı progesteron tedavileri kıyaslandığında PGF2α'nın yeterli cevap oluşturamadığı belirlendi.
SummaryThe efficiency of different progesterone and PGF2α treatments in the induction of ovarian activity and synchronization of oestrus was investigated the beginning transitional period in sheep. A total of 75 ewes were used in the experiment. Animals were divided into 5 groups. Vaginal sponges containing either 30 mg or 40 mg fluorogestene acetate (FGA) were inserted into the vagina of ewes in the first (FGA-30; n=15) and second (FGA-40; n=15) groups, respectively. The sponges were withdrawn after 12 day. In the third group of ewes (n=15), ear implants containing 3 mg norgestomet (N-IMPLANT) were inserted subcutaneously and removed after 9 day. In the fourth group (n=15), Tiaprost tromethamine (PGF2α; 0.294 mg) was intramuscularly injected twice at an interval of 9-d. The control (Control) group is consisted of 15 ewes. After the second PGF2α injections and the withdrawal of the sponges and implants, 600 IU PMSG was injected to all ewes in the FGA-30, FGA-40, N-Implant and PGF2α groups. After the detection of oestrus ewes, they were naturally mated. Oestrus response rates were significantly higher in the groups , and N-IMPLANT (93.3%) than those in the groups PGF2α (53.3%) and Control (26.6%). However, oestrus rates in the group FGA-40 were only significantly higher (86.6%...
The influence of melatonin administration to sperm donors on the freezability of ram semen and enzyme leakage through sperm cells during different steps of the cryopreservation process were evaluated in the breeding and non-breeding season. Melatonin implantation to rams in the breeding season improved post-thaw sperm viability and intact acrosome rates without influencing the motility rate (p < 0.05). Likewise, the post-thaw alkaline phosphatase release through sperm cells was significantly lower in the melatonin-treated group in comparison with untreated controls (p < 0.05). In the non-breeding season, melatonin administration enhanced intact acrosome rates (p < 0.05) and reduced aspartate aminotransferase activity (p < 0.05) post-thaw in the offseason ejaculates. Melatonin implantation twice in the breeding and non-breeding season did not produce any further improvement in the post-thaw sperm parameters in the non-breeding season ejaculates. It was concluded that melatonin administration to sperm donors improved freezability of ram semen collected from these rams and reduced enzyme leakage through sperm cells during cryopreservation.
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