Background: Non-coding RNAs are increasingly investigated and have great potential for diagnose, prognosis and treatment of cancer. Thus, we investigated the possible relation between NF-κB suppressor-NKILA and HSP90, NF-κB and β-catenin molecules in the MCF-7. HSP90 is a compelling stress protein and together with β-catenin and NF-κB molecules it can be responsible for the cancer cell development. However, NKILA is a novel molecule and there is not comprehensive data about it unlike HSP90, β-catenin and NF-κB alone. Therefore, we suggest that there might be a correlation between NKILA and these proteins. Methods and Results: To investigate the NKILA role on these proteins we inhibited the NKILA by using transfection. MCF-7 cells transfected with NKILA-siRNA and incubated for 5 hours. Then, cells were collected and proteins were extracted to be separated in SDS-PAGE. Aforementioned proteins of siRNA transfected group were evaluated by comparing their band intensities with the control group protein bands by immunoblotting. According to this, HSP90 and NF-κB/p105, NF-κB/p65 and NF-κB/p50 subunits significantly increased while β-catenin significantly decreased after NKILA inhibition.Conclusion:, For the first time we demonstrate that HSP90 and beta-catenin is associated with NKILA levels and this may highly related with canonical NF-κB pathway in MCF-7 cells. These novel findings may have important implications in cancer cells development and might present important hints for the future studies about the cancer cell targeted therapy.
We investigate the quantum behavior encountered in palindromes within DNA structure. In particular we reveal the unitary structure of usual palindromic sequences found in genomic DNAs of all living organisms using the Schwinger approach. We clearly demonstrate the role played by palindromic configurations with special emphasis on physical symmetries in particular subsymmetries of unitary structure. We unveil the prominence of unitary structure in palindromic sequences in the sense that vitally significant information endowed within DNA could be transformed unchangeably in the process of transcription. We introduce a new symmetry relation namely purine-purine or pyrimidine-pyrimidine symmetries (p-symmetry) in addition to the already known symmetry relation of purine-pyrimidine symmetries (pp symmetry) given by Chargaff rule. Therefore important vital functions of a living organisms are protected by means of these symmetric features. It is understood that higher order palindromic sequences could be generated in terms of the basis of the highest prime numbers that make up the palindrome sequence number. We propose that violation of this unitary structure of palindromic sequences by means of our proposed symmetries leads to a mutation in DNA which could offer a new perspective in the scientific studies on the origin and cause of mutation.
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