Introduction: The ionizing radiation exposure of the normal cell causes damage to DNA, which leads to cell dysfunction or even cell death. However, it is necessary to identify new radio protectives in order to protect normal cells. Sulindac sulfide (SS) is a metabolite of sulindac (a non-steroidal anti-inflammatory drug) known as a cyclooxygenase inhibitor. Free radicals and reactive oxygen species are generated in the IR-exposed cells. Also, the induced inflammation process causes damage in DNA. Purpose: In this research, the radioprotective effect of SS was investigated against genotoxicity and lipid peroxidation induced by ionizing radiation in the human blood lymphocytes. Methods: In this study, the human blood samples were pretreated with SS at different concentrations (10, 25, 50, 100 and 250 μM) and then were exposed to IR at a dose of 1.5 Gy. The micronucleus (MN) assay was used to indicate the radioprotective effects of SS on exposed cells. Total antioxidant activity of the SS was measured by using FRAP and DPPH assay. Also, the malondialdehyde (MDA) levels and the activity of superoxide dismutase (SOD) on the exposed cells were evaluated. Results: It was found that SS decreased the percentage of MN induced by IR in exposed cells. Maximum reduction in the frequency of MN was observed at 250 μM of SS (87%) that provides the highest degree of protection against IR. On the other hand, pretreatment at 250 μM of SS inhibited IR-induced oxidative stress, which led to a decrease in the MN frequencies and MDA levels, while SOD activity showed an increase in the exposed cells. Conclusion: It could be concluded that SS as a good radioprotective agent protects the human normal cells against the oxidative stress and genetic damage induced by IR.
Introduction: The current study aims to assess the antinociceptive and anti-inflammatory activities of Amygdalus eburnea Spach extract in mice. Methods: Totally, 114 NMRI mice were used in this study. The acute toxicity was evaluated for two days. The antinociceptive effect was accessed by using the hot-plate, tail-flick, and rotarod test. In this investigation, anti-inflammatory effects were evaluated by using the Xylene-induced ear edema method. The findings demonstrated that in the hot-plate and tail-flick tests, A. eburnea extract particularly at the dose of 750 mg/kg demonstrated a considerable analgesic effect; so that there was a significant difference between the extract-treated group and the control group (p<0.05). Results: The results showed that the administration of A. eburnea extract especially at the dose of 500 and 750 mg/kg significantly decreased the ear edema induced by xylene in comparison to the control group. There was no significant difference after injecting of various doses of A. eburnea extract in the sensory-motor test (p > 0.05). Conclusion: These results demonstrated the potent antinociceptive and anti-inflammatory effects of A. eburnea extract in mice. Nevertheless, the precise mechanisms responsible for these activities remain to be studied.
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