Objective: This research aimed to evaluate the effect of fractionation of Merremia mammosa Lour. (Mm (Lour.)) extract on diabetic wound healing by observing collagen synthesis process and to search the most potent fraction.Methods: Wistar rats were divided into five groups (n=5), i.e., K-(negative control), K+ (positive control), K1 (ethyl acetate fraction), K2 (water fraction), and K3 (n-hexane fraction). The Mm (Lour.) was extracted with ethanol 70%, then fractionated by using three solvents which have different polarity. The rats were adapted in 7 days, then induced into diabetic by streptozotocin dose 40mg/kg body weight. The wound was made by Morton excision method. Treatment was given every two days and skin biopsy was done on day 11. Analysis of collagen density was done by photomicrograph of histopathology preparations in Masson’s trichome stained by using trinocular microscope with 400x magnification in 6 fields of view, then processed by imageJ software and analyzed by appropriate statistic tool.Result: The results of this research showed that fractionation of Mm (Lour.) extract significantly enhanced diabetic wound healing based on macroscopic (percentage of wound healing) and collagen density with p-value<0.05 when compared with negative control, especially the water fraction (p=0.000). The follow up post hoc analysis showed that there was no significant (p=0.989) or there was no meaningful difference in the group of water fraction when compared to positive control.Conclusion: Water fraction is the extract fraction of Mm (Lour.) which has the most significant influence on diabetic wound healing showed by enhancement of collagen synthesis.
AbstrakWorld Health Organization (WHO) memperkirakan terdapat 265.000 kematian pada tahun 2014 akibat luka bakar. Luka bakar akan mengakibatkan kerusakan kulit dan juga masalah komplikasi lainnya seperti dehidrasi, infeksi, dan kegagalan sistem organ lainnya. Daun Syzygium samarangense mangandung flavonoid dan saponin yang dapat meningkatkan aktivasi makrofag dan TGF-B yang akan mempercepat proses pembentukan kolagen sehingga proses penyembuhan luka lebih cepat. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun Syzygium samarangense terhadap proses penyembuhan luka bakar berdasarkan kolagen. Penelitian ini menggunakan true experimental design secara in vivo. Luka bakar dibuat dengan cara menempelkan koin selama 10 detik yang sebelumnya dioven selama 5 menit dalam suhu 70 0 C. Rattus Wistar sebagai hewan coba yang dibagi menjadi 6 kelompok (n=4) dengan rincian kelompok A (normal), B (positif), C (negatif
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