A novel, simple, sensitive and stability-indicating reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the quantitative determination of the geometric isomer (Trans) and other related substances in the active pharmaceutical ingredient (API) of Tafluprost (TFL), with their determination by an assay. A chromatographic separation of TFL and its impurities was achieved with a C18 analytical column, using gradient elution with mobile phase A consisting of a mixture of water, methanol and orthophosphoric acid (900:100:1, v/v) and mobile phase B consisting of a mixture of acetonitrile and water (900:100, v/v). The instrumental settings included a flow rate of 1.0 mL/min for related substances and 1.2 mL/min for the assay, a column temperature of 50°C and a detector wavelength of 210 nm, using a photodiode array detector. TFL was exposed to thermal, photolytic, hydrolytic and oxidative stress conditions and the stressed samples were analyzed by the proposed method. Peak homogeneity data of TFL were obtained by using a photodiode array detector in the stressed sample chromatograms, which demonstrated the specificity of the method for estimation in the presence of degradants. The developed method was validated for parameters such as precision, accuracy, linearity, limit of detection, limit of quantification, ruggedness and robustness as per ICH guidelines.

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The present work is aimed at investigating the antimicrobial activity of Lanthanum Potassium Ferrate (LKFO) nanoparticles against P. aeruginosa-a gram-negative bacteria and S. aureus-a gram-positive bacteria. For comparative study, series of LKFO nanoparticles was prepared and assessed for antibacterial efficacy. The average size of the synthesized nanoparticles varied from 20 nm to 100nm and X-ray diffraction pattern showed the formation of a single phase LKFO of an perovskite crystal structure after annealing the precursor at 800 0 C for 8 hr. The antibacterial activity of synthesised nanoparticles were tested on gram positive and gram negative bacteria according to the radial diffusion assay (RDA) for antibacterial agents. The results indicate promising antibacterial activity of La x K 1-x FeO 3 (x= 0.1, 0.2, 0.3) on both gram-positive and gram-negative bacteria. The presence of potassium in the perovskite catalyst offers substantial benefits for surface mobility and electron donor properties. MIC values for S.aureus and P.aeruginosa, were in the range of 50-400 mg/mL, the enhanced antibacterial activity is attributed to increased doping of potassium (K) stoichiometric ratio of the perovskite nanoparticles. MBC studies showed that the growth of S.aureus and P.aeruginosa could be ceased above 100 mg/mL of La x K 1-x FeO 3 (x= 0.1, 0.2, 0.3). Thus the data supports the use of such nanoparticles as a potential antimicrobial agents specially with regards to water purification technology.

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