Momordica dioica is a dioecious cucurbit. The fruits are used as costly vegetable in southern part of India. The biotypes were collected form Warangal and Khammam districts of Andhra Pradesh. When the nodal region were cultured on MS medium supplemented with 2.0 mg/l 6-Benzylaminopurine BAP +2.0 mg/l L-Glutamic acid, the explants produced little amount of callus and shoot buds. The shoot buds on successive subcultures for twice on the same medium produced multiple shoots. Shoot proliferation was further continued even after six months.
An efficient protocol is developed for the induction of shoot regeneration from leaf and node derived callus of Pueraria tuberosa on MS medium supplemented with TDZ (Thidiazuron) (2.0mg/l) and IAA (1.0mg/l) after two of subsequent subcultures. This is the first report of its kind in this plant of Pueraria tuberosa. It is belongs to member of Paplionaceae family. The obtained shoots were elongated after four weeks on the same medium and induced roots by transferring the cultures on half strength MS medium supplemented with 3.0 mg/l IBA after four weeks of inoculation. These plantlets were allowed for hardening and acclimatization for field transfer.
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