The present study was carried out to investigate the correlation coefficient, path analysis and genetic variability among some barley varieties for nine characters in a Randomize Block Design (RBD) with three replications in three environments of Bangladesh. High genotypic coefficient of variation (GCV) was obtained from grain/ spike (29.89 %), yield/ plant (28.72%) and effective tiller/plant (21.86 %) and spike length (13.56 %). The characters with high GCV indicated high potential for selection. The highest heritability (Hb) was observed for 1000 seed weight (95.09) followed by yield/ plant (93.98), grain/ spike (92.09) and spike length (69.93), days to heading (72.65) but the lowest Hb was identified for effective tiller/plant (22.41) followed by the plant height (34.21). Those traits with higher heritability may be considered for selection. Grain/ spike had the highest positive direct effect (5.65) on yield followed by 1000 seed weight (4.65), spike length (1.26), yield/ plant (0.66), days to heading (0.55) and days to maturity (0.34). These parameters were identified as direct selection. Direct negative effect on yield was shown by plant height (-0.32) and effective tiller/plant (-0.74). This was an indication of indirect selection. Int. J. Appl. Sci. Biotechnol. Vol 7(2): 243-247