The study was undertaken to identify genotypic diversity at molecular level of potato for varietal improvement program at the Advanced Plant Breeding Laboratory, Department of Genetics and Plant Breeding, Bongobondhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh. Eight cultivars of potato viz. Lalpakri, Sindurkouta, Indurkani, Ausha, Patnai, Sadaguti, Shilbilati, Challisha were collected from Bangladesh Agricultural Research Institute (BARI), for studying genotyping divergence. Genomic DNA was extracted from young leaves of the cultivars and PCR reaction was performed. The PCR amplified DNA profile was visualized on agarose gel, staining with ethidium bromide. Three RAPD primers named OPA 01, OPA 03 and OPD 18 were used to evaluate the genetic diversity of potato varieties. All the primers were polymorphic and the primers produced the highest number of alleles. The genetic diversity value in Lalpakri with all cultivars except Sadaguti was found to have the highest genetic distance (1.0). The amount of genetic diversity within potato germplasm is quite distinct as revealed by the genetic similarity coefficients. The results indicate that, high level of genetic distance exists among the cultivars. The Primers OPA 01, OPA 03 and OPD 18 showed the highest level of genetic diversity and PIC value while the Lalpakri and Sadaguti cultivars had the highest genetic distance among other cultivars which could be used for further potato breeding program.
Chrysanthemum is the world’s second most economically important flower crop and commonly known as ‘Autumn Queen’. It belongs to the family Compositeae (Asteraceae). It is native to Asia and northeastern Europe and has been cultivated for more than 2000 years. The present study within vitro regeneration of chrysanthemum was carried out to develop the standardized protocol for organogenesis. In this study, three types of explants viz. apical shoot tip, internodal segment and young leaf along with different concentrations and combinations of growth regulators were used for in vitro regeneration. BAP and KIN were used for in vitro microshoot regeneration and IBA along with 2, 4-D were used for in vitro microroot regeneration. Minimum days (7.00) for microshoot initiation, maximum microshoot initiation percentage (97.00), highest number of microshoot per plantlet (12.00), highest number of leaves per microshoot (14.60) and maximum microshoot length (4.60) at 28 DAC were recorded as best performances by apical shoot tip inoculated into MS medium supplemented with BAP 2.5 mg/L + KIN 0.5 mg/L. On the other hand, minimum days (5.00) for microroot initiation, maximum microroot initiation percentage (97.60), the highest number of microroots per plantlet (11.80) and maximum microroot length (6.20) were obtained from apical shoot tip inoculated into ½ strength MS medium supplemented with IBA 0.2 mg/L + 2, 4-D 0.1 mg/L. In case of microshoot regeneration, the best response was showed by apical shoot tip when inoculated into MS medium supplemented with BAP 2.5 mg/L + KIN 0.5 mg/L and the microrooting of plantlets were best from apical shoot tip inoculated into ½ MS medium supplemented with IBA 0.2 mg/L along with 2, 4-D 0.1 mg/L.
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