The tegument of herpesviruses is a highly complex structural layer between the nucleocapsid and the envelope of virions. Tegument proteins play both structural and regulatory functions during replication and spread, but the interactions and functions of many of these proteins are poorly understood. Here we focus on two tegument proteins from herpes simplex virus 1 (HSV-1), pUL7 and pUL51, which have homologues in all other herpesviruses. We have now identified that HSV-1 pUL7 and pUL51 form a stable and direct protein-protein interaction, their expression levels rely on the presence of each other, and they function as a complex in infected cells. We demonstrate that expression of the pUL7-pUL51 complex is important for efficient HSV-1 assembly and plaque formation. Furthermore, we also discovered that the pUL7-pUL51 complex localizes to focal adhesions at the plasma membrane in both infected cells and in the absence of other viral proteins. The expression of pUL7-pUL51 is important to stabilize focal adhesions and maintain cell morphology in infected cells and cells infected with viruses lacking pUL7 and/or pUL51 round up more rapidly than cells infected with wild-type HSV-1. Our data suggest that, in addition to the previously reported functions in virus assembly and spread for pUL51, the pUL7-pUL51 complex is important for maintaining the attachment of infected cells to their surroundings through modulating the activity of focal adhesion complexes.IMPORTANCE Herpesviridae is a large family of highly successful human and animal pathogens. Virions of these viruses are composed of many different proteins, most of which are contained within the tegument, a complex structural layer between the nucleocapsid and the envelope within virus particles. Tegument proteins have important roles in assembling virus particles as well as modifying host cells to promote virus replication and spread. However, little is known about the function of many tegument proteins during virus replication. Our study focuses on two tegument proteins from herpes simplex virus 1 that are conserved in all herpesviruses: pUL7 and pUL51. We demonstrate that these proteins directly interact and form a functional complex that is important for both virus assembly and modulation of host cell morphology. Further, we identify for the first time that these conserved herpesvirus tegument proteins localize to focal adhesions in addition to cytoplasmic juxtanuclear membranes within infected cells.

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The bit error rate performance of a V-Blast encoded 8x8 MIMO MC-CDMA wireless communication system for different signal detection (MMSE and ZF) and digital modulation (BPSK, QPSK, DPSK, and 4QAM) schemes for grayscale image transmission has been investigated in this paper. The proposed wireless system employ ½-rated Convolution and cyclic redundancy check (CRC) channel encoding over the AWGN channel and Walsh Hadamard code as an orthogonal spread code. The present Matlab based simulation study demonstrates that the V-Blast encoded 8×8 MIMO MC-CDMA wireless system with the employment of 1⁄2- rated convolution and cyclic redundancy check (CRC) channel encoding strategies shows good performance utilizing BPSK digital modulation and ZF signal detection scheme in grayscale image transmission.

Digital modulation increases information capacity, data security, and system availability while maintaining high communication quality. As a result, digital modulation techniques are in higher demand than analog modulation techniques due to their ability to transmit larger amounts of data. Amplitude Shift Keying (ASK), Frequency Shift Keying (FSK), Phase Shift Keying (PSK), Differential Phase Shift Keying (DPSK), and Quadrature Amplitude Modulation (QAM) are critical components of current communications systems development, particularly for broadband wireless communications. In this paper, the comparison of bit error rate performance of different modulation schemes (BPSK, QPSK, and16-QAM) and various equalization techniques such as constant modulus algorithm (CMA) and maximum likelihood sequence estimate (MLSE) for the AWGN and Rayleigh fading channels is analyzed using Simulink. BPSK outperforms QPSK and 16-QAM when compared to the other two digital modulation schemes. Among the three digital modulation schemes, BPSK is showing better performance as compared to QPSK and 16-QAM.